常州大学学报（自然科学版）2025，Vol.37Issue(5)：57-64,8.DOI:10.3969/j.issn.2095-0411.2025.05.007
活性污泥中噬菌体的潜在抗生素降解功能
Potential antibiotic degradation function of phage in activated sludge
摘要
Abstract
This study used metagenomic sequencing technology to analyze ordinary activated sludge and sludge enriched with Sulfamethoxazole(SMZ),Sulfadiazine(SDZ)and Amoxicillin(AMX)to explore the function of phages in sludge degradation of antibiotics.Through the qualitative and quanti-tative analysis of phages in sludge samples,it was found that the phages in ordinary activated sludge and antibiotic-enriched sludge were mainly Siphoviridae,Myoviridae and Podoviridae,and there were significant differences among different sludges.Through the functional gene annotation of the phage genome,it was found that the Siphoviridae phage in the AMX-enriched sludge carried the gene encoding Toluene methyl-monooxygenase,and the hydroxylation function of the enzyme played an im-portant role in the degradation of aromatic organics.Based on the metagenomic data,bacterial ge-nomes were reconstructed and phages and functional genes were identified.It was found that the host bacteria of phages had high diversity and had important organics degradation functions.Therefore,bacteria in activated sludge are widely parasitized by phages,and phages may play an important role in the degradation of antibiotics in sludge.关键词
抗生素/生物降解/噬菌体/宏基因组/活性污泥Key words
antibiotic/biodegradation/phage/metagenomics/activated sludge分类
资源环境引用本文复制引用
孙浩浩,王欣雨,许光明,何俊瑜,任艳芳,薛银刚..活性污泥中噬菌体的潜在抗生素降解功能[J].常州大学学报（自然科学版）,2025,37(5):57-64,8.基金项目
江苏省建设系统科研资助项目(2022ZD084) （2022ZD084）
常州市科技支撑计划(社会发展)资助项目(CE20225070) （社会发展）
常州大学科研启动经费资助项目(ZMF23020040). （ZMF23020040）
