检验医学与临床2025，Vol.22Issue(17)：2316-2320,5.DOI:10.3969/j.issn.1672-9455.2025.17.003
支气管哮喘患儿血清CCR5、ADAM8水平与肺功能、气道炎症及哮喘控制情况的关系
Association of serum CCR5 and ADAM8 levels with pulmonary function,airway inflammation and asthma control status in children with bronchial asthma
摘要
Abstract
Objective To investigate the association of serum levels of C-C chemokine receptor type 5(CCR5)and a disintegrin and metalloproteinase 8(ADAM8)with pulmonary function,airway inflammation and asthma control status in children with bronchial asthma.Methods A total of 120 children with bronchial asthma admitted to the hospital from June 2018 to June 2023 were selected as the asthma group,and 115 healthy children who underwent physical examination in the hospital during the same period were selected as the control group.Children with bronchial asthma were classified into the mild group(38 cases),moderate group(47 cases)and severe group(35 cases)according to acute exacerbation severity criteria.After 28 d treatment,asthma patients were stratified into the well-controlled group and poorly-controlled group based on pulmonary function improvement and Childhood Asthma Control Test(C-ACT)scores.Serum CCR5 and ADAM8 levels were measured in all participants.In the asthma group,interleukin-6(IL-6),tumor necrosis factor(TNF)-α,total inflammatory cell count and precentoge of eosinophils in induced sputum were quanti-fied.Pearson correlation was used to analyze the correlation of CCR5 and ADAM8 levels with lung function indexes and airway inflammation related indexes in children with bronchial asthma.The receiver operating characteristic(ROC)curve was used to analyze the value of CCR5 and ADAM8 alone and their combination in predicting poor control of bronchial asthma.Results Serum CCR5 and ADAM8 levels were significantly high-er in the asthma group than those in the control group,with statistically significant differences(P＜0.05).Se-rum CCR5,ADAM8,IL-6 and TNF-α levels,as well as total inflammatory cell count and precentoge of eosino-phils in induced sputum in the severe group were significantly higher than those in the moderate group and mild group,whereas the FEV1/FVC ratio and PEF％were significantly lower,with statistically significant differences(P＜0.05).Serum CCR5,ADAM8,IL-6 and TNF-α levels,total inflammatory cell count and eo-sinophil count in induced sputum in the moderate group were significantly higher than those in the mild group,and FEV1/FVC and PEF％were significantly lower than those in the mild group,with statistically sig-nificant differences(P＜0.05).Pearson correlation analysis showed that serum CCR5 and ADAM8 levels were positively correlated with TNF-α and IL-6 levels,as well as total inflammatory cell count and eosinophil count in induced sputum(P＜0.05),while being negatively correlated with FEV1/FVC and PEF％in children with bronchial asthma(P＜0.05).A total of 94 patients were classified as well-controlled group and 26 as poorly-controlled group.Serum CCR5 and ADAM8 levels were markedly higher in the poorly-controlled group than those in the well-controlled group,with statistically significant differences(P＜0.05).The ROC curve a-nalysis showed that the area under the curve(AUC)for predicting poor asthma control were 0.766(95％CI:0.697-0.852)for CCR5 and 0.792(95％CI:0.708-0.860)for ADAM8.The CCR5 and ADAM8 combined prediction for predicting poor asthma control achieved a significantly higher AUC of 0.905(95％CI:0.837-0.951),which was significantly greater than that of either marker alone(P＜0.05).Conclusion Serum CCR5 and ADAM8 levels are significantly elevated in children with bronchial asthma and are associated with im-paired pulmonary function,airway inflammation and suboptimal asthma control,suggesting their utility in mo-nitoring disease progression.关键词
支气管哮喘/趋化因子受体5/去整合素金属蛋白酶8/肺功能/气道炎症Key words
bronchial asthma/C-C chemokine receptor 5/a disintegrin and metalloproteinase 8/pul-monary function/airway inflammation分类
医药卫生引用本文复制引用
汪莉,梁敏,闫敏..支气管哮喘患儿血清CCR5、ADAM8水平与肺功能、气道炎症及哮喘控制情况的关系[J].检验医学与临床,2025,22(17):2316-2320,5.基金项目
陕西省卫生健康委员会科研基金项目(2021D0020). （2021D0020）
