检验医学与临床2025，Vol.22Issue(17)：2335-2339,2345,6.DOI:10.3969/j.issn.1672-9455.2025.17.006
血清IL-5、sICAM-1联合检测对儿童肺炎支原体感染伴气道高反应的诊断价值
Diagnostic value of combined detection of serum IL-5 and sICAM-1 in children with Mycoplasma pneumoniae infection with airway hyperresponsiveness
摘要
Abstract
Objective To investigate the diagnostic value of combined detection of serum interleukin(IL)-5 and soluble intercellular adhesion molecule-1(sICAM-1)in children with Mycoplasma pneumoniae infection with airway hyperresponsiveness.Methods A total of 174 children with Mycoplasma pneumoniae infection admitted to our hospital from March 2022 to February 2024 were selected as the research objects.According to the results of bronchial provocation test,they were divided into normal airway response group and airway hy-perresponse group.The serum levels of IL-5 and sICAM-1 in all subjects were detected by enzyme-linked im-munosorbent assay.Forced vital capacity(FVC),peak expiratory flow(PEF),forced expiratory volume in one second(FEV1),initial resistance value(Rrs cont)and basal respiratory conduction rate(Grs cont)were measured in all children.Pearson correlation analysis was used to analyze the correlation between serum IL-5,sICAM-1 levels and FVC,PEF,FEV1,Rrs cont,Grs cont in children with airway hyperresponsiveness.Multi-variate Logistic regression was used to analyze the influencing factors of Mycoplasma pneumoniae infection with airway hyperresponsiveness in children.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic value of IL-5,sICAM-1 alone and combined detection in children with Mycoplasma pneumoniae infection with airway hyperresponsiveness.Results There were 94 patients in the normal airway responsiveness group and 80 patients in the airway hyperresponsiveness group.The levels of serum IL-5,sI-CAM-1 and Rrs cont in the airway hyperresponsiveness group were higher than those in the normal airway re-sponse group,and FVC,PEF,FEV1 and Grs cont were lower than those in the normal airway response group,and the differences were statistically significant(P＜0.05).Pearson correlation analysis showed that the ser-um levels of IL-5 and sICAM-1 in the airway hyperresponsiveness group were negatively correlated with FVC,PEF,FEV1 and Grs cont(P＜0.05),and positively correlated with Rrs cont(P＜0.05).The results of multivariate Logistic regression analysis showed that increased levels of serum IL-5 and sICAM-1 were risk factors for the occurrence of airway hyperresponsiveness in children with Mycoplasma pneumoniae infection(P＜0.05).ROC curve analysis showed that the area under the curve(AUC)of serum IL-5,sICAM alone and in combination for the diagnosis of Mycoplasma pneumoniae infection with airway hyperresponsiveness in chil-dren was 0.897,0.901 and 0.994 respectively.The AUC of combined diagnosis was greater than serum IL-5,sICAM alone(Z=4.155,3.177,P＜0.05).Conclusion The serum levels of IL-5 and sICAM-1 in children with Mycoplasma pneumoniae infection and airway hyperresponsiveness are significantly increased and they are related to airway response indicators.The combination of IL-5 and sICAM-1 has a high diagnostic value for Mycoplasma pneumoniae infection and airway hyperresponsiveness in children.关键词
肺炎支原体感染/气道高反应/白细胞介素-5/可溶性细胞间黏附分子-1/诊断价值Key words
Mycoplasma pneumoniae infection/airway hyperresponsiveness/interleukin-5/soluble intercellular adhesion molecule-1/diagnostic value分类
医药卫生引用本文复制引用
李然然,马小龙,代丽莎..血清IL-5、sICAM-1联合检测对儿童肺炎支原体感染伴气道高反应的诊断价值[J].检验医学与临床,2025,22(17):2335-2339,2345,6.基金项目
河北省邯郸市科学技术研究与发展计划项目(24422083080ZC). （24422083080ZC）
