检验医学与临床2025，Vol.22Issue(17)：2346-2351,6.DOI:10.3969/j.issn.1672-9455.2025.17.008
外周血M1/M2巨噬细胞比值及凋亡率与心肌梗死后冠脉微循环功能相关指标和心脏功能指标的相关性
Correlation between M1/M2 macrophage ratio and apoptosis rate in peripheral blood and coronary microcirculation function indicators and cardiac function indicators after myocardial infarction
摘要
Abstract
Objective To investigate the correlation of peripheral blood M1/M2 macrophage ratio and ap-optosis rate with coronary microcirculation function indicators and cardiac function indicators in patients with acute myocardial infarction(AMI)after treatment.Methods A total of 134 AMI patients who underwent percutaneous coronary intervention(PCI)in the hospital from September 2022 to January 2024 were selected as the research subjects.The clinical data of all patients were collected,including gender,age,body mass index(BMI),systolic blood pressure,diastolic blood pressure,fasting blood glucose level and onset time.The pro-portion of M1 and M2 macrophages was detected and the ratio of M1/M2 macrophages was calculated.The level of macrophage apoptosis was detected and the apoptosis rate was calculated.Coronary pressure(Pd),transit time(Tmn),coronary flow(CFR),myocardial flow to microcirculatory resistance ratio(FFR),myo-cardial flow to microcirculatory resistance index(IMR),left ventricular ejection fraction(LVEF),left ventric-ular end-systolic diameter(LVESD)and the ratio of early diastolic to late diastolic mitral flow velocity(E/A)were measured.Patients were divided into normal group and abnormal group according to the presence or ab-sence of coronary microcirculation disturbance(CMD).Pearson correlation analysis was used to analyze the correlation of M1/M2 macrophage ratio and apoptosis rate with CFR,FFR,IMR,LVEF,LVESD and E/A in AMI patients after PCI.Receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of M1/M2 macrophage ratio and apoptosis rate for CMD in AMI patients after PCI.Results There were 97 cases in the normal group and 37 cases in the abnormal group.There was no significant difference in gen-der,age,BMI,systolic blood pressure,diastolic blood pressure,fasting blood glucose level,and onset time be-tween the normal group and the abnormal group(P＞0.05).CFR,FFR,LVEF and E/A in the normal group were higher than those in the abnormal group,and the ratio of M2 macrophages,the ratio of M1 macrophages,M1/M2 macrophages,IMR and LVESD in the normal group were lower than those in the abnormal group,and the differences were statistically significant(P＜0.05).The apoptosis rate of macrophages in the normal group[(9.95+2.03)％]was lower than that in the abnormal group[(13.08+2.94)％],and the difference was statistically significant(t=—7.011,P＜0.05).Pearson correlation analysis showed that the ratio of M1/M2 macrophages in AMI patients after PCI was negatively correlated with CFR,FFR,LVEF and E/A(P＜0.05),and positively correlated with IMR and LVESD(P＜0.05).The apoptosis rate of macrophages in AMI patients after PCI was negatively correlated with CFR,FFR,LVEF and E/A(P＜0.05),and positively corre-lated with IMR and LVESD(P＜0.05).ROC curve analysis showed that the area under the curve of M1/M2 macrophage ratio and apoptosis rate for predicting CMD in AMI patients after PCI was 0.779 and 0.817 re-spectively.Conclusion The ratio of M1/M2 macrophages and apoptosis rate in peripheral blood are correlated with the levels of coronary microcirculation related indicators and cardiac function recovery related indicators after myocardial infarction,and the levels of these indicators can be used to predict the occurrence of CMD in AMI patients after PCI.关键词
心肌梗死/冠脉微循环障碍/心脏功能/巨噬细胞/经皮冠状动脉介入术Key words
myocardial infarction/coronary microcirculation disturbance/cardiac function/macro-phages/percutaneous coronary intervention分类
医药卫生引用本文复制引用
黄宇,张莱,方旭,史培青,陶琴,张郁青..外周血M1/M2巨噬细胞比值及凋亡率与心肌梗死后冠脉微循环功能相关指标和心脏功能指标的相关性[J].检验医学与临床,2025,22(17):2346-2351,6.基金项目
国家自然科学基金项目(82300305). （82300305）
