检验医学与临床2025，Vol.22Issue(18)：2469-2474,6.DOI:10.3969/j.issn.1672-9455.2025.18.004
肺癌合并肺部感染患者病原菌分布及血清miR-124-3p、miR-223的临床意义
Pathogen distribution and clinical significance of serum miR-124-3p and miR-223 in lung cancer patients with pulmonary infection
摘要
Abstract
Objective To investigate the distribution of pathogen distribution and the clinical significance of serum microRNA-124-3p(miR-124-3p)and microRNA-223(miR-223)in patients with lung cancer compli-cated by pulmonary infection.Methods A total of 106 lung-cancer patients complicated by pulmonary infec-tion(infection group)and 106 lung-cancer patients without pulmonary infection(non-infection group)who were treated at the hospital from May 2022 to May 2024 were enrolled as the research subjects.Patients in the infection group were further stratified into the mild group(43 cases)and moderate-severe group(63 cases)based on the Clinical Pulmonary Infection Score(CPIS).Serum miR-124-3p and miR-223 expression levels in all participants were quantified by fluorescence real-time PCR,and multivariate Logistic regression was em-ployed to identify factors associated with infection severity in lung-cancer patients with pulmonary infection.Results A total of 132 pathogenic strains were isolated from sputum samples of the 106 lung cancer patients with pulmonary infection.Gram-negative bacteria(G-bacteria)were the most prevalent(62.12％),primarily Pseudomonas aeruginosa and Escherichia coli.Gram-positive bacteria(G+bacteria)were the next most com-mon(29.55％),predominantly Staphylococcus aureus.Fungi accounted for the smallest proportion(8.33％).Serum miR-124-3p level was significantly lower in the infection group compared with that in the non-infection group(P＜0.05).Conversely,serum miR-223 level was significantly higher in the infection group than that in the non-infection group(P＜0.05).Serum miR-124-3p level was significantly lower in the moderate-severe group than that in the mild group(P＜0.05).Serum miR-223 level was significantly higher in the moderate-severe group compared with that in the mild group(P＜0.05).The proportion of patients with a Karnofsky Performance Status(KPS)score＜80 was significantly higher in the moderate-severe group than that in the mild group(P＜0.05).Fungal and G+bacterial detection rates were significantly higher in the mild group than those in the moderate-severe group(P＜0.05).G-bacterial detection rate was significantly higher in the moderate-severe group than that in the mild group(P＜0.05).A KPS score＜80,elevated serum miR-223 level,and decreased serum miR-124-3p level were identified as independent risk factors for increased infection severity in lung cancer patients with pulmonary infection(P＜0.05).Conclusion G-bacteria were the pre-dominant pathogens in lung cancer patients with pulmonary infection.Decreased serum miR-124-3p level and elevated serum miR-223 level were significantly associated with increased severity of pulmonary infection.关键词
肺癌/肺部感染/微小RNA-124-3p/微小RNA-223/病原菌Key words
lung cancer/pulmonary infection/microRNA-124-3p/microRNA-223/pathogen分类
医药卫生引用本文复制引用
崔雅梅,许笑歌,孙文菲..肺癌合并肺部感染患者病原菌分布及血清miR-124-3p、miR-223的临床意义[J].检验医学与临床,2025,22(18):2469-2474,6.基金项目
河北省廊坊市科学技术研究与发展计划项目(2020013111). （2020013111）
