检验医学与临床2025，Vol.22Issue(18)：2488-2492,5.DOI:10.3969/j.issn.1672-9455.2025.18.007
儿童系统性红斑狼疮患儿外周血IL-2、IL-4、IL-6、IL-10水平与CD4+CD25+Treg比例的相关性分析
Association between peripheral blood IL-2,IL-4,IL-6,IL-10 levels and CD4+CD25+Treg proportion in children with systemic lupus erythematosus
摘要
Abstract
Objective To analyze the correlation between peripheral blood interleukin(IL)-2,IL-4,IL-6,IL-10 levels and CD4+CD25+regulatory T cells(Treg)proportion in children with systemic lupus erythema-tosus(cSLE),and to provide references for clinical application.Methods A total of 114 cSLE children admit-ted to the hospital from June 2022 to June 2024 were enrolled as the cSLE group,while 100 healthy children undergoing physical examinations in the hospital were selected as the non-cSLE group.Correlations between peripheral blood IL-2,IL-4,IL-6,IL-10 levels and CD4+CD25+Treg proportion were analyzed by Pearson cor-relation.Baseline characteristics were collected,and multivariate Logistic regression was performed to identify influencing factors for cSLE development.The receiver operating characteristic(ROC)curve was plotted to e-valuate the auxiliary diagnostic value of peripheral IL-2,IL-4,IL-6,IL-10 and CD4+CD25+Treg proportion for cSLE.Results There were no statistically significant differences between the two groups in terms of age,sex,genetic disease history proportion,prothrombin time,blood potassium level and thyroid-stimulating hormone level(P＞0.05).The levels of IL-2 and the proportion of CD4+CD25+Treg in the cSLE group were lower than those in the non-cSLE group,while the levels of IL-4,IL-6 and IL-10 were higher,and the differences were statistically significant(P＜0.05).Pearson correlation analysis showed that IL-2 level was positively correlated with CD4+CD25+Treg proportion(r=0.267,P＜0.05),whereas IL-4,IL-6 and IL-10 levels were negatively correlated with CD4+CD25+Treg proportion(r=-0.294,-0.337,-0.221,all P＜0.05).Multi-variate Logistic regression revealed that elevated IL-4,IL-6 and IL-10 levels were independent risk factors for cSLE(P＜0.05),whereas higher IL-2 level and a greater CD4+CD25+Treg proportion were protective factors(P＜0.05).The areas under the curve(AUC)for the individual and combined diagnosis of cSLE using pe-ripheral blood IL-2,IL-4,IL-6,IL-10 and CD4+CD25+Treg proportion were 0.957,0.898,0.943,0.763,0.721 and 0.996 respectively.Conclusion Peripheral blood levels of IL-2,IL-4,IL-6 and IL-10 are correlated with of CD4+CD25+Treg proportion in children with cSLE,indicating clinical value for early diagnosis,disease moni-toring and therapeutic assessment.关键词
系统性红斑狼疮/白细胞介素-2/白细胞介素-4/白细胞介素-6/白细胞介素-10/CD4+CD25+调节性T细胞/儿童Key words
systemic lupus erythematosus/interleukin-2/interleukin-4/interleukin-6/interleukin-10/CD4+CD25+regulatory T cell/children分类
医药卫生引用本文复制引用
戴炜,戚畅,熊瑞华,龙元..儿童系统性红斑狼疮患儿外周血IL-2、IL-4、IL-6、IL-10水平与CD4+CD25+Treg比例的相关性分析[J].检验医学与临床,2025,22(18):2488-2492,5.基金项目
湖北省科技计划项目[2022MS(LH)289]. （LH）
