昆明医科大学学报2025，Vol.46Issue(9)：15-22,8.DOI:10.12259/j.issn.2095-610X.S20250902
基于生物信息学和细胞实验探讨APOE在结肠癌耐药中的作用
The Role of APOE in Drug Resistance of Colon Cancer Based on Bioinformatics and Cell Experiments
摘要
Abstract
Objective To evaluate the role and potential mechanism of apolipoprotein E(APOE)in drug resistance of colon cancer by bioinformatic tools and cellular experiments.Methods After downloading the microarray dataset GSE196900 from the GEO database,the online tool GEO2R was used to identify genes that were expressed differently in the drug-resistant and control groups.The differently expressed genes were then examined for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment.The STRING database and Cytoscape software were used to build protein-protein interaction(PPI)networks and find hub genes.Hub genes'predictive significance in colon cancer was further assessed.Western blod and qRT-PCR were used to identify changes in APOE expression,whereas Transwell was used to identify changes in the colon cancer cells'capacity for invasion and migration.Results The analysis of GO and KEGG enrichment revealed that the differential genes derived from the GSE196900 dataset were primarily focused on receptor-ligand activity and cytokine-cytokine receptor interaction pathways.Using the CytoNCA plug-in in Cytoscape software,ten hub genes were obtained through PPI construction.Of these,the prognosis of the patients with colon cancer was negatively correlated with the expression of the APOE gene(P<0.05)and the overexpression of the APOE gene might significantly increase the migration and nvasivenessability of colon cancer cells(P<0.05).Conclusion The increased expression of APOE significantly promotes the migration and invasion ability of colon cancer cells,which may be one of the mechanisms by which APOE gene promotes tumor progression in the patients with colon cancer.关键词
结肠癌/化疗耐药/载脂蛋白E/预后/GEOKey words
Colon cancer/Chemotherapy resistance/Apolipoprotein E/Prognosis/GEO分类
医药卫生引用本文复制引用
舒若,罗华友,宋丽君,高宇,侯艳,张鑫锋,李颖..基于生物信息学和细胞实验探讨APOE在结肠癌耐药中的作用[J].昆明医科大学学报,2025,46(9):15-22,8.基金项目
国家自然科学基金(82060525) （82060525）
