昆明医科大学学报2025，Vol.46Issue(9)：63-71,9.DOI:10.12259/j.issn.2095-610X.S20250907
黄芪厚朴活性成分改善前列腺癌的网络药理研究
Network Pharmacological Study on Active Compounds of Astragalus and Magnolia officinalis Against Prostate Cancer
摘要
Abstract
Objective To comprehensively analyze the mechanism of Astragalus and Magnolia officinalis in treating prostate cancer based on the principles of network pharmacology.Methods Active molecular targets of Astragalus and Magnolia officinalis were predicted using the TCMSP and SwissTargetPrediction databases.Prostate cancer-related targets were screened via Genecards,DisGeNET,and OMIM databases.A"disease-active ingredient-target"network was constructed using Venny software,identifying 69 candidate key target genes.A protein-protein interaction(PPI)network was built using the STRING database,followed by GO and KEGG enrichment pathway analyses performed with R language.Constructing a subcutaneous tumor model in nude mice through in vivo experiments and intervening with active ingredients from Astragalus membranaceus and Magnolia offi-cinalis.Results Molecular docking analysis revealed that active components such as astragaloside IV(MOL000438)and honokiol(MOL000398)exhibited significant binding activity with the key target proteins of prostate cancer,including AKT1,ESR1,PPARG,PTGS2,and SRC.Notably,Honokiol demonstrated a binding energy of-8.7 kcal/mol with estrogen receptor α(ESR1,PDB:1a52),forming stable hydrogen bond interaction with the LEU-391 residue.The in vivo experiments further confirmed that the Astragalus-Magnolia active component group showed smaller subcutaneous xenograft tumor volumes in nude mice as compared to the model group(P<0.05).Immunohistochemical analysis revealed significant downregulation of PPARG and PTGS2 protein expression in tumor tissues(P<0.05).QPCR results indicated that the formula bidirectionally regulated gene expression:pro-apoptotic factor AKT1 was upregulated(P<0.05),while cancer-associated genes PTGS2,PPARG,SRC,and ESR1 were downregulated(P<0.05).Conclusion The combination of Astragalus and Magnolia may exert anti-prostate cancer effects through multi-target and multi-pathway synergistic mechanisms,demonstrating favorable binding activity and therapeutic potential.关键词
网络药理学/黄芪/厚朴/前列腺癌/分子对接/GO/KEGG富集分析Key words
Network pharmacology/Astragalus/Magnolia officinallis/Prostatic cancer/Molecular docking/GO/KEGG enrichment analysis分类
医药卫生引用本文复制引用
任李玥,赵明智,王思捷,刘勤,刘家佳..黄芪厚朴活性成分改善前列腺癌的网络药理研究[J].昆明医科大学学报,2025,46(9):63-71,9.基金项目
云南省教育厅科学研究基金(2022J1174) （2022J1174）
