林业科学2025，Vol.61Issue(9)：138-145,8.DOI:10.11707/j.1001-7488.LYKX20250123
牡丹PoLEC1基因的克隆及其功能
Cloning and Function of PoLEC1 Gene in Paeonia ostii
摘要
Abstract
[Objective]The LEAFY COTYLEDON1(LEC1)gene is a transcription factor specifically expressed in plant embryos.The aim of this study is to explore the molecular mechanism of this gene in regulating high frequency somatic embryogenesis of peony,providing a theoretical basis for constructing an efficient and stable high-quality regeneration system and genetic transformation system of tree peony.[Method]Based on the genome of Paeonia ostii and the transcriptome data from our laboratory,PoLEC1 gene was cloned from P.ostii,and the structure and phylogenetic analysis of its encoded protein was performed.The expression characteristics of PoLEC1 gene in different tissues and embryo development were analyzed by real-time quantitative PCR.PoLEC1 gene overexpression vector was constructed,and Arabidopsis thaliana was genetically transformed by inflorescence impregnation method to obtain T3 transgenic plants,and the phenotype of transgenic plants was observed.[Result]PoLEC1 contained the CBFD_NFYB_HMF domain.Phylogenetic analysis showed that PoLEC1 was closely related to grape(Vitis vinifera)and cocoa(Theobroma cacao),and PoLEC1 gene was highly expressed in early and late embryonic development.It was expressed in all tissues of tree peony,with the highest expression in stems,followed by leaves.PoLEC1 was heterogeneically overexpressed in A.thaliana by Agrobacteria-mediated method.The results showed that there was no significant difference in leaf size between PoLEC1 transgenic plants and wild type plants.The number of leaves of PoLEC1 transgenic plants was slightly more than that of wild type,and PoLEC1 transgenic plants bolted later than wild type.Subcellular localization determined that PoLEC1 was localized in the nucleus and cytoplasm.[Conclusion]PoLEC1 may be involved in somatic embryo development and flowering,providing important genetic resources for molecular breeding of peony.关键词
牡丹/PoLEC1/功能验证/亚细胞定位/体胚发生Key words
Paeonia ostii/PoLEC1/functional validation/subcellular localization/somatic embryogenesis分类
农业科技引用本文复制引用
常艳婷,胡陶,张雪,江泽慧,马艳军,邓雅云,张闻博..牡丹PoLEC1基因的克隆及其功能[J].林业科学,2025,61(9):138-145,8.基金项目
国际竹藤中心基本科研业务费专项资助(1632023009). （1632023009）
