中国海洋大学学报（自然科学版）2025，Vol.55Issue(10)：49-58,10.DOI:10.16441/j.cnki.hdxb.20240064
萨氏海鞘早期胚胎发育的DNA 5mC甲基化特征及功能分析
Characterization of 5mC DNA Methylation During Embryogenesis of the Ascidian Ciona savignyi
摘要
Abstract
We investigated the landscape and function of 5mC DNA methylation in the early embryonic development of the ascidian Ciona savignyi.Whole genome bisulfite sequencing was employed to exam-ine 5mC DNA methylation at different developmental stages,including 32-cell,112-cell,gastrula,and tailbud stages.Our findings revealed that that CpG methylation was the predominant pattern,account-ing for approximately 84.7％～86.4％of methylated cytosines,while CHG and CHH only accounted for about 2.0％～3.0％and 10.7％～12.4％,respectively.Statistical analysis of 5mC DNA methyla-tion levels across these developmental stages revealed significant differences,with a notable decrease from the 32-cell to 112-cell stage.Differential methylation analysis indicated differential methylated genes were enriched in the ubiquitin-protein transferase activity pathway,suggesting a potential role for protein ubiquitination in early embryonic development mediated by 5mC DNA methylation.Treatment of C.savignyi embryos with the 5mC DNA methylation inhibitor 5-azacytidine resulted in developmen-tal abnormalities,implying the function of maintaining 5mC DNA methylation levels for embryonic de-velopment in C.savignyi.Transcriptome analysis of embryos treated with 5-azacytidine revealed sig-nificant differential expression of 925 genes,including 192 upregulated genes and 733 downregulated genes.Gene Ontology analysis indicated that differentially expressed genes were mainly enriched in pathways related to G protein-coupled receptors and DNA-binding transcription factors.Our study es-tablished the 5mC DNA methylation landscape in ascidian embryos and underscores the necessity of maintaining proper 5mC DNA methylation levels for early embryonic development.These findings pro-vide reference data for understanding the origin and function of 5mC DNA methylation modifications.关键词
海鞘/DNA 5mC甲基化/早期胚胎发育/甲基化抑制剂/全基因组亚硫酸氢盐测序Key words
ascidian/5mC DNA methylation/early embryonic development/methylation inhibitor/whole genome bisulfite sequencing分类
生物科学引用本文复制引用
孙琦,李英瑞,隗健凯,董波..萨氏海鞘早期胚胎发育的DNA 5mC甲基化特征及功能分析[J].中国海洋大学学报（自然科学版）,2025,55(10):49-58,10.基金项目
崂山实验室科技创新项目(LSKJ202203001)资助 Supported by the Science & Technology Innovation Project of Laoshan Laboratory(LSKJ202203001) （LSKJ202203001）
