中国海洋大学学报（自然科学版）2025，Vol.55Issue(10)：59-72,14.DOI:10.16441/j.cnki.hdxb.20240103
太平洋亚历山大藻对不同磷源的生理响应研究
Studies on the Physiological Response of the Alexandrium pacificum to Different Phosphorus Sources
摘要
Abstract
At present,the lack of dissolved inorganic phosphorus has become a prominent problem in some sea areas,and has become one of the main limiting factors for phytoplankton growth and repro-duction.Dinoflagellates and other phytoplankton have evolved the survival strategy of dissolved organic phosphorus to maintain life activities,but the selective and utilization mechanism of DOP is still un-clear.Therefore,in order to further explore the utilization of DOP by dinoflagellates,this study took Alexandrium pacificum as the research object,explored its utilization capacity of 59 different phosphor-us sources.The physiological responses to different concentrations of organophosphates(sodium β-glyc-erophosphate,SG-P)and inorganic phosphorus(NaH2PO4)were systematically analyzed.The results showed that there were 48 kinds of phosphorus sources available,among which C—O—P bond and sin-gle ester phosphorus source were preferred,but C—P bond phosphorus source was difficult to use.There was a positive correlation between the maximum algae density and P concentration,and the growth of algae was inhibited obviously under P deficiency.Compared with NaH2PO4,this species has a better affinity for SG-P.Alkaline phosphatase activity(APA)has a negative correlation with the phos-phorus concentration in the external environment,that is,alkaline phosphatase is highly expressed when phosphorus is limited.There was no significant difference between intracellular APA and extracel-lular APA in the phosphorus-rich condition.The results of algal toxin measurements showed that the al-gal strain was dominated by low-toxicity C1 and C2,and the total content of unicellular toxins did not differ significantly between the two phosphorus sources and different phosphorus concentrations,but the content of unicellular toxins increased significantly under phosphorus limitation,and the production of highly Saxitoxin would be more favored in the NaH2PO4 condition.It can be seen that the physiologi-cal response of Alexandrium pacificum to different phosphorus source environments enables it to effec-tively utilize organic phosphorus for growth and reproduction in environments lacking inorganic phos-phorus sources,and this evolutionary strategy makes it more competitive.The results of the study may provide important theoretical references for in-depth exploration of the mechanism of harmful algal bloom outbreaks in certain phosphorus-deprived waters.关键词
太平洋亚历山大藻/溶解态有机磷/溶解态无机磷/磷缺乏/碱性磷酸酶/毒素/有害藻华Key words
Alexandrium pacificum/dissolved inorganic phosphorus(DIP)/dissolved organic phos-phorus(DOP)/phosphorus deficiency/alkaline phosphatase/toxins/harmful algal bloom分类
资源环境引用本文复制引用
孙惠琛,徐溢波,仇依琳,陈晓红,孙鹏飞,白洁,晨曦..太平洋亚历山大藻对不同磷源的生理响应研究[J].中国海洋大学学报（自然科学版）,2025,55(10):59-72,14.基金项目
国家自然科学基金项目(U20A20103)资助 Supported by the National Natural Science Foundation of China(U20A20103) （U20A20103）
