中国海洋大学学报（自然科学版）2025，Vol.55Issue(10)：73-80,8.DOI:10.16441/j.cnki.hdxb.20240223
槐糖脂产生菌降解采油废水的特性研究
Research on the Characteristics of Degradation of Oil Extraction Wastewater by Sophorolipid Producing Bacteria
摘要
Abstract
With most oil fields entering the middle and late stage of exploitation,water flooding has be-come one of the main oil recovery operations,and the discharge of oil production wastewater has been increasing,which not only causes serious environmental pollution,but also leads to waste of water re-sources.Focusing on the resource utilization of oil production wastewater in the process of oilfield ex-ploitation,the feasibility of preparing sophorolipid biosurfactant from oil production wastewater and using it as reinjection water was explored through laboratory simulation experiments with Starmerella bombicola O-13-1 as the production strain.The composition and surface tension of residual crude oil in wastewater before and after degradation were analyzed.The changes of bacterial cell structure were ob-served by transmission electron microscopy.The structure of surfactants was identified by high per-formance liquid chromatography-mass spectrometry(HPLC-MS).The results showed that the degrada-tion rates of oil were 18.0％,16.9％and 11.9％,respectively,in the systems with oil concentrations of 0.8％,0.5％and 0.3％in wastewater within 10 days.The degradation rates of normal alkanes(C17—C28)were 7.0％～12.8％,17.9％～44.0％and 4.5％～27.7％,respectively.During the degradation process,there were obvious lipid contents in S.bombicola cells,the surface tension of the wastewater was greatly reduced,and surfactant substances were produced,consisting of acid-type sophorolipid and lactone-type sophorolipid homologs containing 17-L-hydroxy-octadecenoic acid,octadecadienoic acid or octadecanoic acid substituted by 11 acetyl groups.These results indicated that S.bombicola degrade oil pollutants in wastewater and synthesize sophorolipid biosurfactants,which could be used as reinjection water resources after further treatment.关键词
采油废水/生物强化/假丝酵母菌/生物表面活性剂Key words
oil production wastewater/biological enhancement/starmerella bombicola/biosurfactants分类
能源科技引用本文复制引用
黄金华,李俊峰,宋丹丹,王哲,杨昊,梁生康..槐糖脂产生菌降解采油废水的特性研究[J].中国海洋大学学报（自然科学版）,2025,55(10):73-80,8.基金项目
山东省重大科技创新工程项目(2021CXGC010705)资助 Supported by the Major Science and Technology Innovation Engineering Project of Shandong Province(2021CXGC010705) （2021CXGC010705）
