中国海洋大学学报（自然科学版）2025，Vol.55Issue(10)：101-111,11.DOI:10.16441/j.cnki.hdxb.20240373
好氧阶段不同溶解氧条件下序批式反应器的脱氮性能及宏基因组分析
Nitrogen Removal Performance and Metagenomic Analysis of Sequencing Batch Reactor Under Different Dissolved Oxygen Concentrations at Aerobic Phase
摘要
Abstract
The effects of dissolved oxygen(DO)concentration at aerobic phase on nitrogen removal performance,enzymatic activities,microbial community and nitrogen metabolism functional genes were investigated in a sequencing batch reactor(SBR).With the DO concentration at aerobic phase decreased from 5～6 to 2～3 mg·L-1,the nitrification rates and nitrifying enzymatic activities of SBR activated sludge system gradually decreased,while the denitrification rates and denitrifying enzymatic activities gradually increased.Metagenomic analysis revealed that the microbial community of the activated sludge system changed significantly with the decrease of DO concentration at aerobic phase.The relative abun-dance of typical nitrifying bacteria decreased gradually,while that of multiple denitrifying bacteria in-creased gradually.In the process of nitrogen metabolism,the decrease of DO concentration at aerobic stage inhibited the relative abundance of functional genes and key enzymes related to the nitrification process,but promoted the relative abundance of functional genes and key enzymes related to the denitri-fication process,which explained the reasons for the decreased nitrification performance and the in-creased denitrification performance of SBR from the perspective of genes.According to the Kyoto Ency-clopedia of Genes and Genomes(KEGG)database analysis,the decrease of DO concentration at aerobic phase significantly inhibited the carbon metabolism process of activated sludge system and promoted the process of energy generation and electron transfer to some extent.This study further explored the effect of DO concentration at aerobic phase on biological nitrogen removal process of SBR,and provided a the-oretical basis for the DO parameter setting of SBR.关键词
序批式反应器/溶解氧/硝化/反硝化/微生物群落/氮代谢/功能基因Key words
sequencing batch reactor/dissolved oxygen/nitrification/denitrification/microbial com-munity/nitrogen metabolism/functional genes分类
资源环境引用本文复制引用
田涛涛,张文臣,楚光玉,高畅,王倩芝,王加莹,李伟东,冯兆宇,高孟春..好氧阶段不同溶解氧条件下序批式反应器的脱氮性能及宏基因组分析[J].中国海洋大学学报（自然科学版）,2025,55(10):101-111,11.基金项目
中央高校基本科研业务费专项(201964003)资助 Supported by the Fundamental Research Funds for the Central Universities(201964003) （201964003）
