山西农业科学2025，Vol.53Issue(5)：76-83,8.DOI:10.26942/j.cnki.issn.1002-2481.2025.05.09
基于SNP标记的80份雪茄烟叶种质资源遗传多样性分析
Genetic Diversity Analysis of 80 Cigar Tobacco Germplasm Resources Based on SNP Markers
摘要
Abstract
In order to meet the demand of cigar tobacco production in China,Hainan,Yunnan,Sichuan,and other cigar tobacco producing areas actively introduce foreign cigar tobacco varieties for trial planting.The analysis of genetic diversity of imported varieties is of great significance for the removal of repeated imported germplasm and the efficient utilization of fine cigar tobacco germplasm resources.In this study,a total of 1 212 high quality SNP loci were obtained from 80 cigar tobacco germplasm resources by using 1.5K mGPS liquid chip to conduct whole genome genotype testing.By calculation of polymorphism indexes,it was found that the minimum allele frequency(MAF)ranged from 0.05 to 0.50,and the mean value was 0.19.The polymorphism information content(PIC)ranged from 0.090 5 to 0.375 0,and the mean value was 0.23.There were 18 samples with HET greater than 2%and 3 samples with HET greater than 5%.The genetic diversity of loci among 80 cigar tobacco germplasm resources was low.Admixture software was used to analyze the genetic components and population structure of 80 cigar germplasm resources.The results showed that it was most appropriate to divide the tested materials into 7 subgroups.The gene exchange among the 7 groups was not frequent,the genetic background within the 7 groups was single,and a few materials had mixed genetic background.Additionally,plink software was used to calculate IBS genetic distance,and 3 repeated germplasm were removed by precise identification and the other similar germplasm were accurately distinguished.关键词
SNP分子标记/雪茄烟叶/种质资源/邻接法/Admixture、plink软件/遗传多样性Key words
SNP molecular marker/cigar tobacco/germplasm resources/NJ/Admixture plink software/genetic diversity分类
农业科技引用本文复制引用
杜帅斌,吕洪坤,李媛,向小华,焦芳婵,夏长剑,陈德鑫,郭栋,颜统晶,梁滢玥..基于SNP标记的80份雪茄烟叶种质资源遗传多样性分析[J].山西农业科学,2025,53(5):76-83,8.基金项目
海南省自然科学基金企业人才项目(324QY576) （324QY576）
中国烟草总公司重大科技项目(110202101058(XJ-07) （110202101058(XJ-07）
