首页|期刊导航|世界中医药杂志（英文版）|Polydatin Could Ameliorate Type 2 Diabetes Mellitus?Induced Nonalcoholic Fatty Liver by Suppressing Nuclear Factor Kappa?Light-Chain-Enhancer of Activated B Cells/Nucleotide?Binding Domain,Leucine?Rich Repeat?Containing Receptor, Pyrin Domain?Containing 3 Inflammasome Axis Activation
Polydatin Could Ameliorate Type 2 Diabetes Mellitus?Induced Nonalcoholic Fatty Liver by Suppressing Nuclear Factor Kappa?Light-Chain-Enhancer of Activated B Cells/Nucleotide?Binding Domain,Leucine?Rich Repeat?Containing Receptor, Pyrin Domain?Containing 3 Inflammasome Axis Activation
Jin-Li Luo Ling Zhou Jing-Han Xu Tian-Jiao Liu Li-Yuan Qu Meng Fan Dong-Mei Sun Chun-Li Piao
世界中医药杂志（英文版）2025，Vol.11Issue(3)：372-382,11.
世界中医药杂志（英文版）2025，Vol.11Issue(3)：372-382,11.DOI:10.4103/wjtcm.wjtcm_1_25
Polydatin Could Ameliorate Type 2 Diabetes Mellitus?Induced Nonalcoholic Fatty Liver by Suppressing Nuclear Factor Kappa?Light-Chain-Enhancer of Activated B Cells/Nucleotide?Binding Domain,Leucine?Rich Repeat?Containing Receptor, Pyrin Domain?Containing 3 Inflammasome Axis Activation
摘要
Abstract
Objective:Nonalcoholic fatty liver disease(NAFLD)has a global prevalence of 25%and is the main complication of type 2 diabetes mellitus(T2DM)related liver disease,bringing a serious global burden.Polydatin(PD),a compound from the traditional medical herb,was commonly used to protect and treat liver-related disorders.However,its potential mechanism remains unclear.Materials and Methods:The objective of our research was to explore the hepatoprotective properties and the mechanisms involved with PD in the context of NAFLD induced by T2DM.Both in vivo and in vitro models of T2DM-induced NAFLD and PD intervention were developed using db/db mice and HepG2 cell lines.Histopathological staining,immunofluorescence,Western blotting,enzyme-linked immunosorbent assay,and flow cytometry were conducted to identify the therapeutic effects of PD in treating T2DM-induced NAFLD.Results:The results revealed that PD alleviated inflammatory cytokine levels and markedly reduced T2DM-induced histological alterations of livers.In addition,PD restored alanine aminotransferase,aspartate aminotransferase,and lipid metabolism in db/db mice.Furthermore,PD could ameliorate palmitic acid-induced inflammation and pyroptosis by regulating the expressions of nuclear factor kappa-light-chain-enhancer of activated B cells(NF-κB),p-NF-κB,IKK,p-IKK,nucleotide-binding domain,leucine-rich repeat-containing receptor,pyrin domain-containing 3(NLRP3),ASC,caspase-1,and apoptosis-related proteins.The related pathway inhibitors(Bay 11-7082 and CY-09)and molecular docking analysis further confirmed the effectiveness of PD in treating liver inflammation.Conclusions:Our results indicated that PD could serve as a promising candidate for therapy in addressing NAFLD induced by T2DM,potentially through the inhibition of the NF-κB/NLRP3 inflammasome axis activation within the liver.关键词
Inflammation/lipid metabolism/nonalcoholic fatty liver/nuclear factor kappa-light-chain-enhancer of activated B cells/nucleotide-binding domain/leucine-rich repeat-containing receptor/pyrin domain-containing 3 signaling pathways/polydatin/pyrolysisKey words
Inflammation/lipid metabolism/nonalcoholic fatty liver/nuclear factor kappa-light-chain-enhancer of activated B cells/nucleotide-binding domain/leucine-rich repeat-containing receptor/pyrin domain-containing 3 signaling pathways/polydatin/pyrolysis引用本文复制引用
Jin-Li Luo,Ling Zhou,Jing-Han Xu,Tian-Jiao Liu,Li-Yuan Qu,Meng Fan,Dong-Mei Sun,Chun-Li Piao..Polydatin Could Ameliorate Type 2 Diabetes Mellitus?Induced Nonalcoholic Fatty Liver by Suppressing Nuclear Factor Kappa?Light-Chain-Enhancer of Activated B Cells/Nucleotide?Binding Domain,Leucine?Rich Repeat?Containing Receptor, Pyrin Domain?Containing 3 Inflammasome Axis Activation[J].世界中医药杂志（英文版）,2025,11(3):372-382,11.基金项目
This work was supported by The National Natural Science Foundation of China(grant no.81973813),the Shenzhen Science and Technology Innovation Program(grant no.JCY20190809110015528 and no.JCYJ20220531091409022),the Scientific research project of TCM Bureau of Guangdong Province(grant no.20221329),the China Postdoctoral Science Foundation(certificate no.2023M740783),and the Key Science and Technology Research Projects in Nanhai District,Foshan City in 2022(Nanke[2023]No.20-18). （grant no.81973813）
