水生生物学报2025，Vol.49Issue(10)：77-89,13.DOI:10.3724/1000-3207.2025.2025.0027
有机碎屑对环境DNA监测底栖动物的补充作用:以青弋江为例
THE COMPLEMENTARY ROLE OF DETRITUS IN ENVIRONMENTAL DNA MONITORING OF MACROINVERTEBRATES:A CASE STUDY IN QINGYI RIVER
摘要
Abstract
In order to explore the differences in monitoring macroinvertebrate diversity using environmental DNA(eDNA)technology across different sample types,water and detritus samples were collected from the Qingyi River Basin in May 2023 and analyzed by using traditional morphological method.The results showed that eDNA techno-logy detected 692 operational taxonomic units(OTUs),belonging to 3 phyla,6 classes,64 families,and 158 genera.Specifically,water samples identified 416 macroinvertebrates OTUs,belonging to 3 phyla,5 classes,46 families,and 117 genera,while detritus samples identified 435 macroinvertebrates OTUs,belonging to 3 phyla,6 classes,54 fami-lies,and 122 genera.In contrast,the morphological method detected 66 macroinvertebrate species,belonging to 3 phyla,7 classes,and 42 families.Compared to the morphological method,eDNA technology exhibited significantly higher detection capabilities at the family and genus levels,though morphological method performed better in detect-ing gastropod species.Principal Coordinate Analysis(PCoA)revealed significant differences in the macroinvertebrate community structure between water and detritus samples,with complementary species detection between the two sample types.Linear regression analysis indicated a significant decline in species richness detected by eDNA(combin-ing water and detritus samples)from the headwaters of river to its downstream regions,whereas species richness detected by morphological methods showed no significant variation.In eDNA surveys,water samples are a commonly used sample type;our study demonstrates that detritus can serve as an effective complement to water,laying the foun-dation for the standardized application of eDNA technology.关键词
环境DNA技术/形态学方法/采样策略/底栖动物多样性/青弋江流域Key words
Environmental DNA technology/Morphological method/Sampling strategy/Macroinvertebrate diversity/Qingyi River Basin分类
农业科技引用本文复制引用
石克娜,和雅静,赵永晶,崔永德..有机碎屑对环境DNA监测底栖动物的补充作用:以青弋江为例[J].水生生物学报,2025,49(10):77-89,13.基金项目
国家重点研发计划(2021YKC3200103) （2021YKC3200103）
农业农村部渔业专项(HHDC-2022-05)资助Supported by the National Key Research and Development Program of China(2021YFC3200103) （HHDC-2022-05）
the Special Project of the Ministry of Agriculture and Rural Affairs of the people's republic of China(HHDC-2022-05) （HHDC-2022-05）
