水生生物学报2025，Vol.49Issue(10)：116-125,10.DOI:10.3724/1000-3207.2025.2025.0019
青海湖裸鲤ACSL基因家族的全基因组分析及其盐碱胁迫响应
GENOMIC ANALYSIS OF THE ACSL GENE FAMILY IN THE GYMNOCYPRIS PRZEWALSKII AND ITS RESPONSE TO SALINE-ALKALI STRESS
摘要
Abstract
This study delves into the significance of the long-chain acyl-CoA synthetase(ACSL)gene family in Gymno-cypris przewalskii under saline-alkaline stress,conducting bioinformatics analysis on the genome date and exploring saline-alkaline responses through gene expression detection.The results showed that nine ACSL gene family members encoding proteins ranging from 640 to 834 amino acids.Despite notable differences in gene structure,motif and domain analysis showcased a high level of conservation among these members.Chromosome mapping revealed the dispersion of ACSL gene family members across nine chromosomes(Chr8,13,15,40,41,48,64,82,and 87),all encoding hydrophilic properties.Except for ACSL2 and ACSL3a,the rest are stable proteins.The product of ACSL4b is an acidic protein,while all others are basic.Subcellular localization prediction results indicated that ACSL2 is localized to the cytoplasm,ACSL3a,ACSL3b,ACSL4a,and ACSL4b to the peroxisome,and the rest to the cell membrane.qRT-PCR analysis revealed tissue-specific expression patterns of ACSL genes in Gymnocypris przewalskii,with ACSL3b,ACSL4b,and ACSL5 being highly expressed in the kidney,while ACSL3a,ACSL4a,and ACSL5 exhibited elevated expression in the intestine.Under saline-alkali stress,high level expression of ACSL2,ACSL3a,and ACSL6 was detected in the gill,and transcription of ACSL1a,ACSL2,ACSL3a,and ACSL5 were markedly up-regulated in the kidney.In the intestine,expressions of ACSL3b and ACSL6 were prominently up-regulated.These findings highlight the ACSL gene family in Gymnocypris przewalskii contributes to multiple physiological functions under saline-alkaline stress,including energy supply through lipid metabolism,maintenance of osmotic balance,and immune regulation.关键词
长链酰基辅酶A合成酶/盐碱胁迫/基因家族/青海湖裸鲤Key words
Long-chain acyl-CoA synthetase/Saline-alkali stress/Gene family/Gymnocypris przewalskii分类
生物科学引用本文复制引用
张玉璟,崔妍蓉,颜鲁杨,张罗丹,王统刚,魏姝瑾,卫福磊,俞录贤,梁健..青海湖裸鲤ACSL基因家族的全基因组分析及其盐碱胁迫响应[J].水生生物学报,2025,49(10):116-125,10.基金项目
国家自然科学基金(31960741)资助Supported by the National Natural Science Foundation of China(31960741) （31960741）
