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TurboID-based mapping of organelle membrane protein interactomes with digitonin-permeabilization

Yang Sun Lan Yang Jingzi Zhang Pu Tian Shue Chen Lei Fang Zhi Hong

生物物理学报（英文）2025，Vol.11Issue(4)：219-231,13.

生物物理学报（英文）2025，Vol.11Issue(4)：219-231,13.DOI:10.52601/bpr.2025.240051

TurboID-based mapping of organelle membrane protein interactomes with digitonin-permeabilization

Yang Sun ¹Lan Yang ²Jingzi Zhang ³Pu Tian ¹Shue Chen ⁴Lei Fang ³Zhi Hong¹

作者信息

1. Centre for Cellular Biology and Signaling,Zhejiang University-University of Edinburgh Institute,Haining 314400,Zhejiang,China||University of Edinburgh Medical School,Biomedical Sciences,College of Medicine & Veterinary Medicine,Univer-sity of Edinburgh,Edinburgh,EH8 9JZ,United Kingdom
2. Centre for Cellular Biology and Signaling,Zhejiang University-University of Edinburgh Institute,Haining 314400,Zhejiang,China
3. State Key Laboratory of Pharmaceutical Biotechnology,Chemistry and Biomedicine Innovation Center,Medical School of Nanjing University,Nanjing 210000,China
4. International Center for Aging and Cancer,Hainan Medical University,Haikou 571199,China
折叠

摘要

关键词

Digitonin-permeabilization/Organelle interactomes/Proximity labeling/Protein-protein interactions/REEP1/REEP6

Key words

Digitonin-permeabilization/Organelle interactomes/Proximity labeling/Protein-protein interactions/REEP1/REEP6

引用本文复制引用

Yang Sun,Lan Yang,Jingzi Zhang,Pu Tian,Shue Chen,Lei Fang,Zhi Hong..TurboID-based mapping of organelle membrane protein interactomes with digitonin-permeabilization[J].生物物理学报（英文）,2025,11(4):219-231,13.

基金项目

The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE[1]partner repository with the dataset identifier PXD059120.This study was supported by a grant from the National Natural Science Foundation of China(32070700).Open Access funding is provided by University of Edinburgh. （32070700）

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