时珍国医国药2025，Vol.36Issue(17)：3220-3227,8.DOI:10.70976/j.1008-0805.SZGYGY-2025-1704
地五养肝胶囊含药血清活化Nrf2/HO-1信号通路调控巨噬细胞M2极化的机制研究
Study on the mechanism of Diwu Yanggan Capsule (地五养肝胶囊)-containing serum in regulating macrophage M2 polarization through activating the Nrf2/HO-1 signaling pathway
摘要
Abstract
Objective To investigate the effect and mechanism of Diwu Yanggan Capsule(地五养肝胶囊,DWYG)-containing serum on M1/M2 polarization of macrophages.Methods LPS-induced RAW 264.7 cells were divided into the blank control group,model group,DWYG low/medium/high-dose groups.RT-PCR was applied to detect the mRNA expression levels of iNOS,Arg-1,TNF-α,IL-6,IL-10,Nrf2,HO-1,and Keap1 in cells from different groups.ELISA kits were used to measure the contents of TNF-α,IL-6,and IL-10 in the cell culture supernatants from different groups.As for cells from different groups,flow cytometry was employed to detect the intracellular ROS levels from different groups;Colorimetry was utilized to assess the GSH/GSSG ratio in cells from different groups;Western blot(WB)was performed to examine the protein expression levels of Nrf2,HO-1,Keap1,and intranuclear Nrf2.Re-sults DWYG-containing serum intervention decreased mRNA expression levels of iNOS,TNF-α and IL-6 in LPS-induced RAW264.7 cells,as well as decreased the protein secretion levels of TNF-α and IL-6.Conversely,it increased the mRNA expression levels of Arg-1 and IL-10,and enhanced IL-10 protein secretion.Furthermore,DWYG-containing serum intervention reduced in-tracellular ROS levels but elevated the intracellular GSH/GSSG ratio in LPS-induced RAW264.7 cells.Meanwhile,it down-regulated the mRNA and protein expression levels of Keap1,up-regulated the mRNA and protein expression levels of Nrf2 and HO-1,and pro-moted the intranuclear Nrf2 protein expression in LPS-induced RAW264.7 cells.Conclusion DWYG drug-containing serum exerts anti-inflammatory effects by activating the Nrf2/HO-1 signaling axis to regulate macrophage redox status to promote M2 polarization of macrophages.关键词
地五养肝胶囊/巨噬细胞/M1/M2极化/Nrf2/HO-1信号通路/氧化还原Key words
Diwu Yanggan Capsule(地五养肝胶囊)/Macrophage/M1/M2 polarization/Nrf2/HO-1 signaling pathway/Redox分类
医药卫生引用本文复制引用
邓大秀,陈尹滢,毛禹凡,付学良,周煜,李瀚旻,沈昕..地五养肝胶囊含药血清活化Nrf2/HO-1信号通路调控巨噬细胞M2极化的机制研究[J].时珍国医国药,2025,36(17):3220-3227,8.基金项目
国家自然科学基金面上项目(82174344) （82174344）
湖北省自然科学基金联合基金项目(2024AFD244 （）
2024AFD318) （）
中国博士后科学基金面上资助项目(2024M750854) （2024M750854）
