时珍国医国药2025，Vol.36Issue(17)：3240-3246,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-1707
四物五子丸含药血清对过氧化氢诱导的人视网膜色素上皮细胞氧化损伤的保护作用
Protective effect of Siwu Wuzi Pill(四物五子丸)containing serum on hydrogen peroxide-in-duced oxidative damage in ARPE-19 cells
摘要
Abstract
Objective To observe the protective effect of Siwu Wuzi Pill(四物五子丸,SWWZP)containing serum on hydrogen perox-ide(H2O2)-induced oxidative damage in human retinal pigment epithelial(ARPE-19)cells.Methods H2O2 induced the oxidative damage in ARPE-19 cells,which were then assigned to the blank control group,model control group,and SWWZP-containing serum intervention group(further divided into low,medium,and high dose groups);MTT method,scratch test and colorimetric method were used to detect MDA and CAT;Western blot(WB)was used to detect the expressions of Cat B,Cys C,MMP-2 and TIMP-2 proteins;and PCR was used to detect the expressions of Cat B,Cys C,MMP-2 and TIMP-2 mRNA.Results Compared with the blank control group,H2O2 at a concentration of 200 μmol/L formed oxidative damage to ARPE-19 cells for 24 h(P＜0.05);SWWZP-containing serum at concentrations of 2.5％,5％and 10％had a proliferation-promoting effect on ARPE-19 cells(P＜0.05),and the serum intervention group increased the viability of ARPE-19 cells(P＜0.05),decreased the MDA content(P＜0.001),increased the CAT activity(P＜0.05),and significantly decreased the high expression levels of Cat B,Cys C,MMP-2,and TIMP-2(P＜0.05),and significantly down-regulated the high expression levels of Cat B,Cys C,MMP-2,and TIMP-2 mRNA(P＜0.01).Conclusion SWWZP-containing serum was able to increase the antioxidant capacity of ARPE-19 cells,thus exerting a protective effect on ARPE-19 cells.关键词
ARPE-19细胞/氧化损伤/四物五子丸/年龄相关性黄斑变性Key words
ARPE-19 cells/Oxidative damage/Siwu Wuzi Pill(四物五子丸)/Age-related macular degeneration分类
医药卫生引用本文复制引用
谢雨燕,黄冰林,魏含蕾,黄琴,王安琪,李家明,郭亲上,唐秋雨,彭媛,袁灵梅..四物五子丸含药血清对过氧化氢诱导的人视网膜色素上皮细胞氧化损伤的保护作用[J].时珍国医国药,2025,36(17):3240-3246,7.基金项目
国家自然科学基金青年基金(82104935) （82104935）
江西省中医药管理局科技计划项目(2024B134) （2024B134）
