时珍国医国药2025，Vol.36Issue(17)：3247-3256,10.DOI:10.70976/j.1008-0805.SZGYGY-2025-1708
麻杏石甘汤含药血清对阿霉素诱导H9C2心肌细胞损伤的保护作用
Protective effect of serum containing Maxing Shigan Decoction(麻杏石甘汤)on doxorubicin-induced injury in H9C2 cardiomyocytes
摘要
Abstract
Objective To investigate the protective effect of Maxing Shigan Decoction(麻杏石甘汤,MXSGD)on Doxorubicin(DOX)-induced H9C2 cardiomyocyte injury.Methods The potential mechanism of MXSGD in preventing and treating DOX-induced myocar-dial injury was predicted using network pharmacology and molecular docking.A DOX-induced H9C2 cell injury model was established and intervened with MXSGD-contained serum.Cell viability,apoptosis,and morphological changes were detected using MTT and Ho-echst staining assays.The activities of LDH and SOD,and the level of MDA in cells were measured using the assay kits.The protein ex-pression levels of Bcl-2,Bax,and Caspase-3 were detected by Western blot(WB).Results Network pharmacology results indicated that MXSGD primarily involves reactive oxygen species(ROS),IL-17 signaling pathway,apoptosis,tumor necrosis factor signaling pathway,and cell cycle-related signaling pathways.Molecular docking results showed that the components with the best docking scores to CASP3,CASP8,and BCL2 were formononetin,glycyrrhetinic acid,and quercetin,respectively.Intervention with MXSGD-con-tained serum significantly increased the viability of DOX-induced cells(P＜0.01),reduced the apoptosis rate(P＜0.01),de-creased LDH activity and MDA levels(P＜0.01),increased SOD activity(P＜0.01),downregulated Caspase-3 and Bax protein expression(P＜0.01),and upregulated Bcl-2 protein expression(P＜0.01).Conclusion MXSGD-contained serum had the pro-tective effect on DOX-induced cardiomyocytes,possibly through exerting antioxidant effects and inhibiting cardiomyocyte apoptosis.关键词
麻杏石甘汤含药血清/阿霉素/心肌损伤/细胞凋亡Key words
Maxing Shigan Decoction(麻杏石甘汤)-contained serum/Doxorubicin/Myocardial injury/Apoptosis分类
医药卫生引用本文复制引用
刘小瑞,王斯琦,苏敬,杨丽丽,任珂,刘东玲..麻杏石甘汤含药血清对阿霉素诱导H9C2心肌细胞损伤的保护作用[J].时珍国医国药,2025,36(17):3247-3256,10.基金项目
国家自然科学基金面上项目(82074419) （82074419）
甘肃省杰出青年基金(23JRRA1203) （23JRRA1203）
