时珍国医国药2025，Vol.36Issue(17)：3257-3264,8.DOI:10.70976/j.1008-0805.SZGYGY-2025-1709
加减藿朴夏苓汤对厌食模型大鼠脑肠肽及肠道菌群的影响
The effects of modified Huopu Xiangling Decoction(藿朴夏苓汤)on brain-gut peptides and intestinal microbiota in anorexia model rats
摘要
Abstract
Objective To investigate the effects of modified Huopu Xiangling Decoction(藿朴夏苓汤,HPXLD)on brain-gut pep-tides and intestinal microbiota in anorexia model rats.Methods Forty SD rats were randomly divided into the blank control group(10 rats)and the modeling group(30 rats).After successful model establishment using the etiological modeling method,the modeling group was randomly divided into the model group,positive control group,and the treatment group,with 10 rats in each group.The treatment group and the positive control group received corresponding drugs,while the blank control group and the model group were given equal volumes of physiological saline.All groups were administered by gavage for 14 consecutive days,during which the 24-hour food intake and body weight were monitored.Western blot(WB)analysis was performed to detect the expression of hypothalamic neuropeptide Y(NPY)and cholecystokinin-8(CCK-8)proteins.16S rRNA gene sequencing was employed to analyze changes in intestinal microbio-ta.Results Modified HPXLD significantly increased the 24-hour food intake and body weight of anorexia model rats,increased NPY protein expression,and decreased CCK-8 protein expression.Compared with the blank control group,the model group exhibited de-creased relative abundance of Actinobacteria and Romboutsia(among eight other genera),and increased relative abundance of Pro-teobacteria and Veillonella.Compared with the model group,the treatment group showed decreased relative abundance of Bacteroidetes,Papillibacter,and Prevotellaceae_Ga6A1_group,and increased relative abundance of Firmicutes,Actinobacteria,and Roseburia(among ten other genera).Conclusion Modified HPXLD increased food intake and body weight in anorexia model rats,and its mechanism may be associated with the regulation of brain-gut peptide expression and modulation of intestinal microbiota.关键词
儿童厌食/加减藿朴夏苓汤/脑肠肽/肠道菌群Key words
Anorexia in children/Modified Huopu Xiangling Decoction(加减藿朴夏苓汤)/Brain gut peptides/Intestinal mi-crobiota分类
医药卫生引用本文复制引用
彭真,李卉,刘建忠,王文广..加减藿朴夏苓汤对厌食模型大鼠脑肠肽及肠道菌群的影响[J].时珍国医国药,2025,36(17):3257-3264,8.基金项目
湖北省"楚天英才计划"医疗卫生人才项目(鄂卫发[2024]1号) （鄂卫发[2024]1号）
湖北省中医药管理局中医药重点学科建设项目中医儿科学(鄂中医通[2023]2号) （鄂中医通[2023]2号）
湖北省卫生计生委中医药科研项目(ZY2019Q018) （ZY2019Q018）
湖北中医药大学"十四五"优秀学科团队建设项目刘建忠教授团队(100504090209) （100504090209）
