数字中医药（英文）2025，Vol.8Issue(3)：400-412,13.DOI:10.1016/j.dcmed.2025.09.011
hsa-miR-125a-5p与hsa-miR-125b-5p作为监测针灸治疗类风湿性关节炎潜在生物标志物的计算机评估研究
In silico evaluation of hsa-miR-125a-5p and hsa-miR-125b-5p as potential biomarkers for monitoring acupuncture treatment in rheumatoid arthritis
摘要
Abstract
Objective To perform an in silico bioinformatics analysis to identify differentially expressed microRNAs(miRNAs)implicated in rheumatoid arthritis(RA)pathogenesis and evaluate their potential as biomarkers for assessing therapeutic efficacy and monitoring acupuncture treatment. Methods miRNA microarray data(CEL and TXT formats)were acquired from human and murine RA models,with the latter undergoing acupuncture treatment.Data were normalized using the robust multi-array average(RMA)method and analyzed for differential expression.Differential expression analysis identified miRNAs through a comparative analysis of RA hu-man tissues,acupuncture-treated murine RA models,and a bibliographic search for miRNAs implicated in RA pathogenesis and acupuncture treatment.Bioinformatics analysis was per-formed to identify potential target genes for each miRNA and signaling pathways via search tools for the Retrieval of Interacting Genes/Proteins(STRING)and ShinyGO.Gene-drug in-teraction analysis was performed through Drug-Gene Interaction Database(DGIdb)screen-ing.Interaction networks were constructed with the Cytoscape v3.10.3 software. Results The hsa-miR-125a-5p and hsa-miR-125b-5p were identified as potential biomarkers associated with RA pathogenesis,presenting 468 and 455 target genes,respectively.These genes were enriched in 20 signaling pathways,including Janus kinasa-signal transducer and activator of transcription(JAK-STAT),mitogen-activated protein kinase(MAPK),phospho-inositide 3-kinase-protein kinase B(PI3K-Akt),and nuclear factor kappa-light-chain-en-hancer of activated B cells(NF-κB)signaling pathway,which have been associated with RA pathogenesis and progression.Drug-gene interaction networks revealed that 22 genes were significantly associated with 58 RA treatment drugs,among which 13 genes interacted with members of the hsa-miR-125 family. Conclusion The hsa-miR-125a-5p and hsa-miR-125b-5p demonstrate critical regulatory role in RA pathogenesis by modulating signaling pathways,including JAK-STAT,MAPK,PI3K-Akt,and NF-κB.Our findings show that the hsa-miR-125a-5p and hsa-miR-125b-5p exhibit differ-ential expression patterns in response to pharmacological intervention in various diseases,including RA management.This suggests their potential roles as biomarkers for monitoring acupuncture treatment.Although existing evidence indicates that acupuncture can modify miRNA expression profiles,rigorous validation through biological models remains essential to confirm these results.关键词
针灸/类风湿性关节炎/软骨/软骨细胞/生物标志物/生物信息学/miRNAKey words
Acupuncture/Rheumatoid arthritis/Cartilage/Chondrocytes/Biomarkers/Bioinformatics/miRNA引用本文复制引用
Xavier Anaya-Reza,Martha Alicia Ballinas-Verdugo,José Eduardo Justo-Frausto,Sergio Rafael Carrillo-Patiño,Juan Fernando Montes-García,Nu-bia Denise Nieto-Vargas,Rogelio Frank Jiménez-Ortega..hsa-miR-125a-5p与hsa-miR-125b-5p作为监测针灸治疗类风湿性关节炎潜在生物标志物的计算机评估研究[J].数字中医药（英文）,2025,8(3):400-412,13.基金项目
Programa de Investigadores e Investigadoras of the Con-sejo Mexiquense de Ciencia y Tecnología(COMECYT)(EESP2024-0038). （COMECYT）
