数字中医药（英文）2025，Vol.8Issue(3)：413-424,12.DOI:10.1016/j.dcmed.2025.09.012
山香圆叶治疗糖尿病并发症的化学成分及治疗潜力
Shanxiangyuanye(Turpiniae Folium)for diabetic complications:chemical constituents and therapeutic potential
摘要
Abstract
Objective To analyze the chemical constituents of Shanxiangyuanye(Turpiniae Folium)through liquid chromatography-tandem mass spectrometry(LC-MS/MS)method,and to evaluate their anti-oxidant,hypoglycemic,and anti-glycation activities related to diabetic complications. Methods The supernatant of Shanxiangyuanye(Turpiniae Folium)(TFS),obtained follow-ing water extraction and alcohol precipitation,was analyzed by LC-MS/MS.Antioxidant activ-ity of TFS in vitro was evaluated using three experimental approaches:the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging assay,the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS+)radical cation decolorization assay,and the hydroxyl(·OH)radical scavenging assay.To comprehensively evaluate hypoglycemic potential,α-glucosidase inhibi-tion was measured to analyze in vitro hypoglycemic activity.Subsequently,in vitro models were developed to examine anti-glycation activity through the bovine serum albumin(BSA)-fructose(Fru),BSA-methylglyoxal(MGO),BSA-glyoxal(GO),and D-arginine(Arg)-MGO sys-tems,with particular attention to the inhibitory effects of TFS.Furthermore,the concentra-tions of fructosamine,protein carbonyls,sulfhydryl groups,and β-amyloid in the glycation so-lution were quantified using the BSA-Fru model following 7-d of incubation at 37 ℃. Results Using LC-MS/MS analysis in both positive and negative ion modes,we identified 750 chemical components in TFS,primarily including organic acids,amino acids,and their derivatives.In vitro activity studies demonstrated that TFS exhibited remarkable free radical scavenging capacity,with half-maximal inhibitory concentrations(IC50)of 0.47,1.56,and 0.36 mg/mL against DPPH,ABTS+,and·OH radicals,respectively.Regarding hypoglycemic activity,TFS dose-dependently inhibited α-glucosidase activity(IC50=0.21 mg/mL),display-ing comparable efficacy to the clinical drug acarbose(IC50=0.23 mg/mL).Notably,TFS inter-vened in the glycation process:IC50 values were 0.22,1.91-4.96,and 4.09 mg/mL in the BSA-Fru,BSA-MGO/GO,and Arg-MGO models,respectively,with the most prominent inhibitory effects observed in the BSA-Fru model.Furthermore,although TFS may not effectively pre-serve thiol groups in BSA or reduce thiol oxidation during glycation,it significantly reduces fructosamine levels(in a dose-dependent manner),decreases β-amyloid formation,and in-hibits protein carbonylation(P<0.000 1). Conclusion The findings demonstrate that TFS exhibits a complex chemical composition with potent antioxidant,hypoglycemic,and anti-glycation activities.These results provide compelling scientific evidence supporting TFS's potential as a natural adjuvant for diabetes prevention and complication management,while laying a solid foundation for its applica-tions in functional food development and adjunctive antidiabetic therapeutics.关键词
山香圆叶/糖尿病并发症/抗糖基化/降血糖/抗氧化剂Key words
Shanxiangyuanye(Turpiniae Folium)/Diabetic complications/Anti-glycation/Hypoglycemic/Anti-oxidant引用本文复制引用
熊瑞瑶,陈双,戴子豪,龚力民..山香圆叶治疗糖尿病并发症的化学成分及治疗潜力[J].数字中医药（英文）,2025,8(3):413-424,12.基金项目
Key Project of Hunan Provincial Education Bureau(23A0307),and Natural Science Foundation of Hunan Province(2025JJ80089). （23A0307）
