天津农业科学2025，Vol.31Issue(9)：45-49,54,6.DOI:10.3969/j.issn.1006-6500.2025.09.008
几种有机氮源对阿维菌素发酵效价的影响研究
Study on the Effects of Several Organic Nitrogen Sources on the Fermentation Potency of Avermectin
程曦 1杨柳 1李娜 1张月强 1姚清国2
作者信息
- 1. 河北兴柏农业科技股份有限公司河北省阿维菌素生物技术重点实验室,河北石家庄 051530
- 2. 石家庄学院化工学院,河北石家庄 050000
- 折叠
摘要
Abstract
To investigate the effects of several organic nitrogen sources on the fermentation potency of avermectin and explore methods for reducing the production cost of avermectin.Parallel experiments in 50 L fermenters were conducted to prove their effects on potency by replacing soybean cake powder and yeast powder with peanut meal,replacing soybean cake powder with soybean meal powder,replacing starch with com meal,and replacing soybean cake powder with animal peptone.The results showed that:peanut meal at concentrations of 12.8 g·L-1 and 5.8 g·L-1was used to replace soybean cake powder and yeast powder,the average final titers were reduced by 40％and 22％compared to the control,with significant reductions(P=6.1×10-5).Soybean meal powder at 23 g·L-1 was used to completely replace soybean cake powder,resulting in an average final potency increased by 9％compared to the control group.However,after adding 6％soybean oil,the average fermentation potency was 32 μg·g-1 lower than the control group.There was no significant difference between the two group(P=0.92).Starch and soybean cake powder were replaced with corn meal,at concentrations of corn meal(60 g·L-1),starch(98 g·L-1)and soybean cake powder(17 g·L-1 or 20 g·L-1),resulting in an average final potency 12％lower than the control group,showing a significant decrease(P=0.016).Animal peptone at 4 g·L-1 was used as an equivalent substitute for soybean cake powder,resulting in a final potency 21％lower than the control group.In conclusion,soybean meal powder,when used as a replacement for soybean cake powder,can increase the avermectin fermentation potency and reduce production cost.Peanut meal adversely affects avermectin biosynthesis,when its negative impact becoming increasingly detrimental at higher concentrations,therefore,it is not suitable for use in the fermentation medium.The strategy of replacing starch with corn meal to bypass starch processing and lower costs is not viable.Animal peptone disrupts mycelial aggregation,thereby reducing fermentation potency.It is not recommended as a substitute for soybean cake powder in avermectin fermentation.关键词
有机氮源/阿维菌素/发酵效价/影响Key words
organic nitrogen source/avermectin/fermentation potency/effect分类
化学化工引用本文复制引用
程曦,杨柳,李娜,张月强,姚清国..几种有机氮源对阿维菌素发酵效价的影响研究[J].天津农业科学,2025,31(9):45-49,54,6.
