摘要
Abstract
Objective To explore the mechanism of action of Bailing tablets(BLT)in the treatment of vitiligo with nework pharmacology methods.Methods The effective active compounds and target genes of BLT were retrieved,collected,and screened u-sing the TCMSP(Traditional Chinese Medicine System Pharmacology Database and Analysis Platform)and UniProt database platform.The related pathogenic genes of vitiligo were queried through the DisGeNET,OMIM,and GeneCards databases,and the potential com-mon targets for the treatment of vitiligo by BLT were further explored.The data of the active components and action target of BLT were collected and sorted,and then imported into Cytoscape 3.10.2 software to construct an"interaction network of Bailing tablet-active compounds-target genes".The"protein-protein interaction(PPI)core network visualization analysis of Bailing tablet-key targets of vit-iligo"was constructed using the STRING database and Cytoscape software.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on the potential targets of BLT in the treatment of vitiligo using the DAVID database.Results A total of 27 active compounds of main components of BLT,220 drug targets,1,500 disease targets,and 74 overlapping targets were screened out.The network diagram of"Chinese medicine-active compounds-target"showed that the core active compounds of the main components of BLT might be quercetin,kaempferol,7-O-methylisomucronulatol,formononetin,and β-sitosterol.The PPI network was constructed for the key targets of Bailing tablet-vitiligo,and it was found that the key target genes for BLT to treat vitiligo included protein kinase B(AKT1),tumor necrosis factor(TNF),interleukin 6(IL6),tumor protein p53(TP53),interleukin-1β(IL1B),caspase 3(CASP3),B lymphocyte tumor-2 protein(BCL2),human estrogen receptor(ESR1),prostaglandin oxygenase 2(PTGS2),peroxisome prolifera-tor-activated receptor γ(PPARG),etc.The GO functional enrich-ment analysis yielded 602 terms,including 495 biological processes,38 cellular components,and 69 molecular functions.The KEGG pathway enrichment analysis yielded 155 signaling pathways,mainly involving cancer-related pathways,lipid and atherosclerosis,fluid shear stress and atherosclerosis,the AGE-RAGE signaling pathway in diabetes complications,cell aging,human T-cell leukemia virus type 1 infection,the IL-17 signaling pathway,etc.Conclusions BLT may treat vitiligo through multiple components,multiple targets,and multiple pathways.关键词
白灵片/白癜风/作用机制/网络药理学/中药Key words
Bailing tablet/vitiligo/mechanism of action/network pharmacology/traditional Chinese medicine分类
医药卫生
