西安工程大学学报2025，Vol.39Issue(5)：29-38,10.DOI:10.13338/j.issn.1674-649x.2025.05.004
基于低共熔溶剂合成UiO-66-Ser固定化双酶催化制备稀有人参皂苷CK
Catalytic preparation of rare ginsenosides CK based on deep eutectic solvent synthesis of UiO-66-Ser immobilized dual enzymes
摘要
Abstract
Aimed at the environmental pollution problems of toxic solvents(DMF/DMSO)and a-cidic modulators in the traditional synthesis system of Zr-MOFs,a green synthesis strategy based on choline-based deep eutectic solvent(DES)was developed in this study.The UiO-66-Ser carrier material was successfully constructed by introducing the biocompatible modulator molecule L-ser-ine in place of the conventional acidic medium,and the synergistic snailase and β-glucosidase were covalently crosslinked to the carrier,synthesizing the biocomposite Sna@UiO-66-Ser@β-G for the targeted catalytic conversion of ginsenoside Rb1 into the rare ginsenoside CK.Through the one-way experiments,the preparation conditions of UiO-66-Ser and Sna@UiO-66-Ser@β-G biocom-posites were optimized:L-Ser addition 1.5 g,reaction temperature 140℃,cross-linking time 4 h,snailase:40 mg,and β-glucosidase:150 μL.Optimization of the catalytic reaction conditions indica-ted that under the conversion time of 48 h,pH 4.0 and the optimal process conditions of enzyme-substrate ratio of 1∶2,the conversion of ginsenoside Rb1 into the rare ginsenoside CK was a-chieved,and the yield of the target product rare ginsenoside CK reached 87%.This study provides a novel catalytic system and process technology solution for the efficient conversion of natural product active ingredients.关键词
低共熔溶剂/蜗牛酶/β-葡萄糖苷酶/人参皂苷Rb1/稀有人参皂苷CK/金属有机框架Key words
deep eutectic solvent/snailase/β-glycosidase/ginsenoside Rb1/rare ginsenoside CK/metal-organic frameworks分类
生物科学引用本文复制引用
王晓军,崔春晓,武占省..基于低共熔溶剂合成UiO-66-Ser固定化双酶催化制备稀有人参皂苷CK[J].西安工程大学学报,2025,39(5):29-38,10.基金项目
国家重点研发计划(2021YFC2101500) （2021YFC2101500）
陕西省高校青年科技创新团队(2022TD071) （2022TD071）
