厦门大学学报（自然科学版）2025，Vol.64Issue(5)：809-817,9.DOI:10.6043/j.issn.0438-0479.202404028
Cu-ZIF-8金属有机框架纳米材料的合成及抗菌性能
Synthesis and antibacterial properties of Cu-ZIF-8 metal organic framework nanomaterials
摘要
Abstract
[Objective]Bacterial infections pose a critical threat to global public health.Metal-organic frameworks(MOFs),a novel class of porous biodegradable materials,have attracted considerable attention in the field of antibacterial research.They offer several advantages,including low production costs,high design flexibility,structural diversity,simple preparation processes,water solubility,and long-term stability.These properties position MOFs as an attractive solution for pioneering advancements in antibacterial treatments,providing a sustainable alternative to traditional antibiotic therapies and addressing the urgent challenge of antibiotic resistance.However,MOFs often rely solely on a single mechanism of action,which can significantly limit their antibacterial efficacy.To enhance their therapeutic potential,it is essential to develop MOFs with synergistic mechanisms.[Methods]In this study,we applied encapsulation and coordination techniques to introduce additional functionalities to zeolitic imidazolate framework-8(ZIF-8)MOFs,which are ideal candidate of antibacterial agents due to their good affordability,mild synthesis conditions,ease of functionalization,and potential antimicrobial activities.Our team successfully synthesized Cu-ZIF-8 nanoparticles utilizing a straightforward,one-pot method conducted at room temperature.These nanoparticles were characterized by a series of analytical methods including transmission electron microscopy(TEM),energy dispersive spectroscopy(EDS),X-ray diffraction(XRD),and X-ray photoelectron spectroscopy(XPS).Their responsiveness to acidosis and light was assessed using TEM and electron spin resonance(ESR)spectroscopy,and their minimum inhibitory mass concentration(MIC)and minimum bactericidal mass concentration(MBC)against Staphylococcus aureus and Escherichia coli were evaluated with serial dilution assays.Their antibacterial mechanism was further investigated with confocal laser scanning microscopy(CLSM).Finally,these nanoparticles were used to prepare antibacterial textile fabrics and the efficacy of these fabrics against S.aureus was studied.[Results]ESR spectroscopy analysis revealed that Cu-ZIF-8 nanoparticles were capable of generating reactive oxygen species(ROS)even under dark conditions,with production substantially enhanced upon exposure to sunlight.This elevated ROS level initiated oxidative stress,leading to notable antibacterial effects.Additionally,Cu-ZIF-8 nanoparticles released antibacterial Zn2+and Cu2+in mildly acidic environments,enhancing their bactericidal effectiveness through synergistic mechanisms.In vitro testing demonstrated that these nanoparticles exhibited potent antibacterial activity against both S.aureus and E.coli,with M ICs of 31.25 and 300.0 μg/mL under light condition,respectively.Notably,textiles treated with Cu-ZIF-8 nanoparticles showed significant antibacterial efficacy against S.aureus,highlighting their practical applications.These results confirm the dual functionality of Cu-ZIF-8 nanoparticles and their promise as antibacterial biomaterials with significant potential in medical and environmental applications.The enhanced antibacterial activity under light conditions introduces new possibilities for designing novel antibacterial materials that leverage both natural and artificial light sources,offering effective,light-responsive solutions to combat microbial infections.[Conclusion]Cu-ZIF-8 nanoparticles were successfully synthesized using a straightforward one-pot method at room temperature,demonstrating their substantial antibacterial efficacy.These nanoparticles not only exhibited significant inhibitory effects on different bacterial strains but also provided key data and theoretical insights to support the further development of efficient antibacterial materials.This work represents a meaningful advance in antibacterial research.Moving forward,future studies will focus on evaluating antibacterial effects of these nanoparticles under diverse environmental conditions and optimizing their practical applications across different domains.关键词
沸石咪唑酯骨架-8(ZIF-8)/双重响应/抗菌活性/活性氧物种Key words
zeolitic imidazolate framework(ZIF-8)/dual-responsive/antibacterial activity/reactive oxygen species分类
通用工业技术引用本文复制引用
李京燕,熊楠,樊一凡,林泓域,高锦豪..Cu-ZIF-8金属有机框架纳米材料的合成及抗菌性能[J].厦门大学学报（自然科学版）,2025,64(5):809-817,9.基金项目
国家自然科学基金(22077107) （22077107）
福建省自然科学基金(2023J06002) （2023J06002）
