湖北科技学院学报（医学版）2025，Vol.39Issue(5)：380-385,6.DOI:10.16751/j.cnki.2095-4646.2025031308
棕榈酸通过抑制AMPK-Nrf2/HO-1轴诱导大鼠心肌细胞铁死亡
Palmitic Acid Induced Ferroptosis in Rat Cardiomyocytes by Inhibiting the AMPK-Nrf2/HO-1 Axis
摘要
Abstract
Objective To investigate the role of adenylate activated protein kinase(AMPK)and its mechanism on palmitic acid(PA)-induced ferroptosis in rat cardiomyocytes.Methods The H9c2 cells were treated with PA for 24 hours,and the effect of PA on cell vi-ability was detected by CCK-8.The expression levels of AMPK,Nrf2,HO-1,and ferroptosis-related factors were detected by RT-qPCR and Western blot.And the lipid peroxidation level was detected by lipid peroxidation assay kit.Further pretreatment with AMPK agonist AICAR or inhibitor Compound C was performed to evaluate the changes in the above indicators.Meanwhile,iron assay kit,MDA kit and GSH assay kit were utilized to detect the changes in the content of iron ions,MDA and GSH in each group,respectively.Results PA increased the con-tent of LPO and decreased the protein and gene expression levels of AMPK,Nrf2,HO-1,ferroptosis-related factors SLC7A11 and GPX4.However,pretreatment with the AMPK agonist AICAR reversed PA-induced cell damage,increased cellular GSH levels,and decreased MDA and iron levels.In contrast,the AMPK inhibitor Compound C further exacerbated the damage,decreased the cellular GSH level,and in-creased MDA and iron levels(P＜0.05).Conclusion PA induces ferroptosis in rat cardiomyocytes by modulating the Nrf2/HO-1 signaling pathway through down-regulation of AMPK.关键词
棕榈酸/腺苷酸激活蛋白激酶/铁死亡/Nrf2/HO-1信号通路Key words
Palmitic acid/Adenylate activated protein kinase/Ferroptosis/Nrf2/HO-1 signaling pathway分类
医药卫生引用本文复制引用
凌莉,华玉,季慧敏,朱薿,肖钰杰,姜敏生,任展宏,王定淼..棕榈酸通过抑制AMPK-Nrf2/HO-1轴诱导大鼠心肌细胞铁死亡[J].湖北科技学院学报（医学版）,2025,39(5):380-385,6.基金项目
糖尿病与血管病变专项项目(2022TNB04) （2022TNB04）
湖北科技学院科研创新团队项目(2022T01) （2022T01）
