湖北科技学院学报（医学版）2025，Vol.39Issue(5)：386-390,5.DOI:10.16751/j.cnki.2095-4646.2025010610
白桦脂酸通过下调c-Myc抑制宫颈癌SIHA细胞的增殖与迁移
Betulinic Acid Inhibits the Proliferation and Migration of Cervical Cancer SIHA Cells Through Downregulation of c-Myc Protein
祝巧云 1朱波2
作者信息
- 1. 咸宁市咸安区中医医院妇产科,湖北咸宁 437000
- 2. 湖北科技学院医学部基础医学院人体解剖学教研室
- 折叠
摘要
Abstract
Objective To evaluate the inhibitory effect and partial molecular mechanism of betulinic acid(BA)on the malignant bi-ological behavior of cervical cancer SIH A cells.Methods Cervical cancer SIH A cells were cultured in cell incubator with constant tempera-ture and humidit and treated with different concentration gradients of BA to detecte the proliferation and migration ability.The optical-density of SIHA cells was detected by MTT assay,and then the time-dependent inhibition of SIHA cells by BA at the same concentration was evalua-ted.The single cell clonal proliferation ability of SIHA cells was detected by plate clone formation assay.The migration and invasion ability of SIHA cells were evaluated by Transwell cell small chamber migration and invasion assay.The c-Myc protein expression level in SIHA cells treated with BA was deteced by Western blot assay.Resue assay was applied to demonstrate whether the inhibitory effect of BA was mediated by c-Myc pathway.Results BA could inhibit the survival and proliferation of SIHA cells in a time and dose-dependent manner,as well as the migration and invasion in a dose-dependent manner.In a certain dose range,BA down-regulated the expression of oncogene c-Myc protein.c-Myc-saRNA can partly counteract the inhibition effect of BA.Conclusion BA can inhibit the proliferation and migration of cervical cancer SIHA cells,and its mechanism is related to the down-regulation of c-Myc protein expression.关键词
白桦脂酸/宫颈癌/SIHA/c-Myc/迁移Key words
Betulinic acid/Cervical cancer/SIHA/c-Myc/Migration分类
医药卫生引用本文复制引用
祝巧云,朱波..白桦脂酸通过下调c-Myc抑制宫颈癌SIHA细胞的增殖与迁移[J].湖北科技学院学报（医学版）,2025,39(5):386-390,5.
