湖北科技学院学报（医学版）2025，Vol.39Issue(5)：396-400,405,6.DOI:10.16751/j.cnki.2095-4646.2025010714
三氟拉嗪二盐酸盐对前列腺癌的作用机制研究
A Study on the Mechanism of Trifluoperazine Dihydrochloride in Prostate Cancer
摘要
Abstract
Objective To use network pharmacology,molecular docking technology,and cell experiments to deeply explore the mech-anism of trifluoperazine dihydrochloride on prostate cancer.Methods The targets of trifluoperazine dihydrochloride and prostate cancer-relat-ed targets were screened out through the database and a visual network and protein-protein interaction network(PPI)were constructed,fol-lowed by GO and KEGG enrichment analysis was carried out,and finally,the potential targets were verified by molecular docking software.In the cell experiment,prostate cancer cells were treated with trifluoperazine dihydrochloride at different concentration gradients.The MTT col-orimetric method and plate clone formation experiment were used to verify its inhibitory effect on cell proliferation.The scratch experiment and transwell invasion and migration experiment were employed to demonstrate the intervention effect on the invasion and migration charac-teristics of cancer cells.Results The results of network pharmacology analysis showed that trifluoperazine dihydrochloride has 70 targets in-volved in the fight against prostate cancer.KEGG enrichment analysis further revealed that the drug can exert its significant anti-prostate cancer effect through multiple key signal pathways such as cancer-related pathways and PI3K-AKT pathway.The molecular docking results showed that trifluoperazine dihydrochloride has an extremely ideal binding activity with the core targets.And its inhibitory effect on the vitali-ty,proliferation,migration and invasion of prostate cancer cells showed a distinct dose-dependent characteristic.Conclusion Trifluoperazine dihydrochloride may inhibit prostate cancer cell proliferation through multiple targets,and the inhibitory ability is proportional to the dose.关键词
三氟拉嗪二盐酸盐/前列腺癌细胞/增殖/网络药理学/分子对接Key words
Trifluoperazine dihydrochloride/Prostate cancer cell/Proliferation/Network pharmacology/Molecular docking分类
医药卫生引用本文复制引用
谢孟婷,刘艳,贾爱亭,蔡敬,刘复兴,宁志丰..三氟拉嗪二盐酸盐对前列腺癌的作用机制研究[J].湖北科技学院学报（医学版）,2025,39(5):396-400,405,6.基金项目
湖北省重点研发计划大健康专项(2022BCE011) （2022BCE011）
