湖北科技学院学报（医学版）2025，Vol.39Issue(5)：416-421,6.DOI:10.16751/j.cnki.2095-4646.2025030706
PVA/PSBMA/AgNPs两性离子水凝胶伤口敷料的辐射制备及其抗菌抗污性能研究
Radiation Preparation of PVA/PSBMA/AgNPs Amphoteric Hydrogel Wound Dressings and its Antibacterial and Antifouling Properties
摘要
Abstract
Objective Using electron beam irradiation to prepare multifunctional medical zwitterionic hydrogels with antibacterial and anti-biofouling properties along with biocompatibility,aiming to meet the application demands of biomedical materials in wound dressings and other fields.Methods A two-step irradiation technique was used to pre-polymerize methacryloyloxyethyl sulfobetaine(SBMA)into poly(sul-fobetaine methacrylate)(PSBMA).Using poly(vinyl alcohol)(PVA)and PSBMA as matrices,silver nanoparticles(AgNPs)were intro-duced to prepare the PVA/PSBMA/AgNPs hydrogel through a combination of freeze-thaw cycles and electron beam radiation crosslinking.The component structure,elemental distribution,and interaction mechanisms were characterized through FTIR and XPS analyses so as to fur-ther evaluate the properties including gel fraction,swelling ratio,hydrophilicity,in vitro antibacterial activity,anti-biofouling efficacy,and bio-compatibility.Results At a radiation dose of 20 kGy,the PVA/PSBMA hydrogel achieved a gel fraction of 84.04％,indicating optimal crosslinking efficiency.When loaded with 3 mL of AgNPs solution,the hydrogel exhibited optimal antibacterial performance along with favor-able swelling capacity and hydrophilicity.Additionally,the protein adsorption was as low as 4.38 μg/cm2;the MTT assay showed cell viabili-ty above 90％,and live/dead cell staining revealed no significant cytotoxicity.Conclusion The zwitterionic PVA/PSBMA/AgNPs composite hydrogel prepared through the two-step radiation technique has good antibacterial efficacy,robust anti-biofouling properties,and excellent biocompatibility,and is expected to become a novel high-performance wound dressing.关键词
水凝胶/辐射制备/聚乙烯醇/聚甲基丙烯酰乙基磺基甜菜碱/纳米银粒子Key words
Hydrogel/Radiation preparation/Polyvinyl alcohol/Poly methacryloyloxyethyl sulfobetaine/Silver nanoparticles分类
医药卫生引用本文复制引用
周士忠,张洋林,唐冬旭,刘坤,李月生..PVA/PSBMA/AgNPs两性离子水凝胶伤口敷料的辐射制备及其抗菌抗污性能研究[J].湖北科技学院学报（医学版）,2025,39(5):416-421,6.基金项目
国家自然科学基金(11405050) （11405050）
湖北省自然科学基金联合创新基金重点项目(2025AFD387) （2025AFD387）
湖北省高等学校优秀中青年科技创新团队项目(T2020022) （T2020022）
武汉儿童医院博士启动基金(2024FEBSJJ006) （2024FEBSJJ006）
湖北科技学院科技发展基金(2020TD01、2022HX104) （2020TD01、2022HX104）
