眼科新进展2025，Vol.45Issue(10)：799-804,6.DOI:10.13389/j.cnki.rao.2025.0137
增生型糖尿病视网膜病变伴玻璃体视网膜异常粘连患者的玻璃体液代谢特征
Metabolic characteristics of vitreous fluid in patients with proliferative dia-betic retinopathy with abnormal vitreoretinal adhesion
摘要
Abstract
Objective A non-targeted metabolomics analysis of vitreous fluid from patients with proliferative diabetic retinopathy(PDR)is conducted to explore the"metabolic map"of PDR.This approach aims to deepen the understanding of the disease,identify potential biomarkers.Methods From 35 PDR patients and 30 fresh rhegmatogenous retinal de-tachment(RRD)patients,10 PDR patients with vitreoretinal abnormal adhesions were selected as the experimental group(PDR group),and 10 fresh RRD patients were chosen as the control group(RRD group).Using ultra-high-performance liq-uid chromatography-mass spectrometry non-targeted metabolomics technology,the metabolic profiles of vitreous fluid were analyzed to obtain metabolic spectra.One-dimensional and multidimensional statistical methods were used to analyze the differences in metabolites and metabolic pathways between the PDR and RRD groups.Results A total of 165 differential metabolites were identified in the vitreous humor samples of patients in the PDR and RRD groups,these differential metab-olites were significantly enriched in 21 metabolic pathways(P＜0.05),Among these pathways,those with at least 5 differ-ential metabolites include:methionine and cysteine metabolism;glycine,serine,and threonine metabolism;ascorbic acid and aldose metabolism;amino acid biosynthesis;and central carbon metabolism in cancer.Pyruvate,serine,D-2-phospho-glycerate,threonine,phosphoserine,and high serine are present in multiple metabolic pathways,the areas under the curve are 0.96,0.82,0.85,0.78,0.40,and 0.31,respectively.Conclusion There are 21 significantly different metabolic pathways between PDR and RRD patients.Pyruvate stands out in multiple pathways,potentially serving as a biomarker for PDR diagnosis.关键词
增生型糖尿病视网膜病变/玻璃体视网膜异常粘连/非靶向代谢组学/代谢通路Key words
proliferative diabetic retinopathy/abnormal vitreoretinal adhesion/non-targeted metabolomics/metabol-ic pathway分类
医药卫生引用本文复制引用
黄晓峰,李雨蔓,郭泰,莫芷欣,池铭偲,刘越,孟倩丽,崔颖,黄中宁..增生型糖尿病视网膜病变伴玻璃体视网膜异常粘连患者的玻璃体液代谢特征[J].眼科新进展,2025,45(10):799-804,6.基金项目
国家自然科学基金项目(编号:82000897) （编号:82000897）
广东省基础与应用基础研究基金项目(编号:2024A1515010933) （编号:2024A1515010933）
