Abstract
Objective To observe the expression of tumor protein 53(P53),cytokeratin 5/6(CK5/6),and human epidermal growth factor receptor 2(HER-2)in breast cancer tissues and analyze their associations with pathological features,as well as to evaluate survival curves based on different expression levels.Methods A total of 120 breast cancer patients who underwent radical resection in Gongyi People's Hospital from January 2021 to September 2023 were selected.Immunohistochemistry was used to detect the expression of P53,CK5/6,and HER-2 in breast cancer tissues and adjacent noncancerous tissues.The associations of P53,CK5/6,and HER-2 expression with clinicopathological characteristics were analyzed.Kaplan-Meier curves were used to calculate survival curves according to the expression status of P53,CK5/6,and HER-2.Results The positive expression rates of P53,CK5/6,and HER-2 were higher in breast cancer tissues than in adjacent tissues(P＜0.05).Compared with P53-negative patients,P53-positive patients showed statistically significant differences in age,tumor size,lymph node metastasis,and vascular tumor thrombus(P＜0.05),while there were no significant differences in TNM stage,perineural invasion,or vascular invasion(P＞0.05).Compared with CK5/6-negative patients,CK5/6-positive patients had significant differences in histological grade and lymph node metastasis(P＜0.05),but not in age,tumor size,TNM stage,perineural invasion,vascular invasion,or vascular tumor thrombus(P＞0.05).Compared with HER-2-negative patients,HER-2-positive patients had significant differences in age,histological grade,lymph node metastasis,vascular invasion,and vascular tumor thrombus(P＜0.05),but not in tumor size,TNM stage,or perineural invasion(P＞0.05).Spearman correlation analysis showed that positive P53 expression was positively correlated with lymph node metastasis(r=0.268,P=0.003)and vascular tumor thrombus(r=0.225,P=0.014).Positive CK5/6 expression was positively correlated with high-grade histological differentiation(poorly differentiated)(r=0.429,P＜0.001),lymph node metastasis(r=0.265,P=0.003),vascular invasion(r=0.272,P=0.003),and vascular tumor thrombus(r=0.218,P=0.016).Kaplan-Meier curves showed that patients with positive expression of P53,CK5/6,or HER-2 had shorter overall survival compared with negative patients(P＜0.05).Conclusion The expression of P53,CK5/6,and HER-2 is associated with invasive features and poor prognosis of breast cancer.Combined detection may have clinical reference value,but this conclusion requires confirmation through studies with larger sample sizes and more rigorous designs.关键词
乳腺癌/肿瘤蛋白P53/细胞角蛋白5/6/人表皮生长因子受体2Key words
breast cancer/tumor protein P53/cytokeratin 5/6/human epidermal growth factor receptor 2分类
医药卫生
