中国病理生理杂志2025，Vol.41Issue(9)：1694-1702,9.DOI:10.3969/j.issn.1000-4718.2025.09.004
白细胞介素13通过调控大鼠血管平滑肌表型转化参与血管内膜增生
Interleukin-13 is involved in vascular intimal hyperplasia by regulation of vascular smooth muscle phenotypic transformation
摘要
Abstract
AIM:To investigate the mechanism by which interleukin-13(IL-13)influences vascular smooth muscle cell(VSMC)phenotypic transformation and subsequently contributes to vascular intimal hyperplasia in rats.METHODS:A total of 32 male SD rats,aged 5～7 weeks and weighing 330～360 g,were randomly divided into 4 groups(n=8 per group):normal(Nor)group,normal treatment(Nor+IL-13 neutralizing antibody,Nor+IL-13Nab)group,inju-ry(Inj)group,and injruy treatment(Inj+IL-13Nab)group.A 2F balloon catheter was used to induce mechanical injury in the left common carotid artery of SD rats to establish a vascular intimal hyperplasia model.Hematoxylin-eosin staining was performed to observe vascular structural changes.Enzyme-linked immunosorbent assay(ELISA)kits were used to measure IL-13 and transforming growth factor-β1(TGF-β1)levels.Human aortic smooth muscle cells(HA-SMCs)were cultured in vitro.Flow cytometry was conducted to assess peripheral blood CD4+IL-13+T cell content.Real-time quantita-tive PCR(RT-qPCR)was employed to evaluate gene expression levels of α-smooth muscle actin,osteopontin,calponin,collagen type Ⅰ/Ⅲ,proliferating cell nuclear antigen and Ki-67 antigen.Transwell and scratch wound assays were per-formed to assess cell migration.RESULTS:Compared with the model group,administration of IL-13Nab significantly in-hibited vascular intimal hyperplasia induced by mechanical vascular injury by antagonizing high plasma IL-13 levels(P<0.01).Immunofluorescence and mRNA analysis showed that neutralizing high plasma IL-13 suppressed collagen accumu-lation(P<0.01)and VSMC phenotypic transformation(P<0.01)in the injured vessels but did not inhibit peripheral blood CD4+IL-13+T cell activation.Incubation of HA-SMCs with recombinant human IL-13(rhIL-13)promoted cell pro-liferation and migration(P<0.01)as well as phenotypic transformation(P<0.01).Additional evidence suggested that rhIL-13-induced HA-SMC phenotypic transformation was associated with the regulation of TGF-β1 secretion by HA-SMCs.CONCLUSION:Interleukin-13 promotes vascular intimal hyperplasia by regulating VSMC phenotypic transformation through TGF-β1 secretion in rat models.关键词
白细胞介素13/血管内膜增生/转换生长因子β1/血管平滑肌细胞/表型转换Key words
interleukin-13/vascular intimal hyperplasia/TGF-β1/vascular smooth muscle cells/phenotyp-ic transformation分类
医药卫生引用本文复制引用
武欣,刘逍,张嘉莹,甄子怡,李琦,陈畅..白细胞介素13通过调控大鼠血管平滑肌表型转化参与血管内膜增生[J].中国病理生理杂志,2025,41(9):1694-1702,9.基金项目
黑龙江省重点研发计划(No.2023ZX06C13) （No.2023ZX06C13）
国家自然科学基金面上项目(No.81970382) （No.81970382）
