中国病理生理杂志2025，Vol.41Issue(9)：1713-1720,8.DOI:10.3969/j.issn.1000-4718.2025.09.006
雾化吸入丹参酮ⅡA磺酸钠对小鼠慢性阻塞性肺疾病相关肺动脉高压的治疗作用
Sodium tanshinone ⅡA sulfonate inhalation attenuates pulmonary hyper-tension associated with chronic obstructive pulmonary disease in mice
摘要
Abstract
AIM:To investigate the effects of nebulized sodium tanshinone ⅡA sulfonate(STS)in a mouse model of pulmonary hypertension associated with chronic obstructive pulmonary disease(COPD-PH).METHODS:A to-tal of 32 healthy SPF-grade male C57BL/6 mice were randomly divided into 4 groups:control(CTL,n=8)group,COPD-PH(CS+LPS,n=8)group,STS-treated COPD-PH(CS+LPS+STS,n=8)group,and STS(n=8)group.The COPD-PH model was established through whole-body exposure to cigarette smoke(CS)combined with lipopolysaccharide(LPS)in-halation.Mice were subjected to cigarette smoke exposure in a chamber(9 cigarettes/h,2 h/session,2 sessions/d,6 d/week)for 60 d,except on days of LPS inhalation.On days 1 and 14,COPD-PH model mice received LPS(7.5 µg/mouse in 50 µL saline)via intranasal inhalation,while the CTL and STS groups received an equivalent volume of saline.STS was administered via nebulized inhalation(5 mg/kg,30 min per session,twice daily)immediately before CS exposure.At the end of the modeling period,lung function and right heart pressure were assessed.Bronchoalveolar lavage fluid(BALF)was collected for inflammatory cell counting.Levels of interleukin-6(IL-6)in BALF supernatants and plasma were measured using ELISA.Pathological changes in the airway and lung tissues were evaluated.RESULTS:(1)Com-pared to CTL mice,those exposed to CS and LPS exhibited lesions characteristic of COPD-PH,including emphysema,lung inflammation,decreased lung function,and increased right ventricular systolic pressure(RVSP)and right ventricu-lar hypertrophy index(RVHI)(P<0.05);(2)COPD-PH mice showed significantly elevated IL-6 levels in both BALF and plasma(P<0.05);(3)STS treatment alleviated emphysema and lung inflammation,improved lung function,prevent-ed increases in RVSP and the RV/(LV+S)ratio,and reduced IL-6 levels in both BALF and plasma(P<0.05).CON-CLUSION:The results indicate that nebulized inhalation of STS significantly slows the progression of COPD-PH,likely due to its ability to inhibit lung inflammation and reduce IL-6 expression in the lungs.关键词
丹参酮ⅡA磺酸钠/慢性阻塞性肺疾病/肺动脉高压/白细胞介素6/炎症Key words
sodium tanshinone ⅡA sulfonate/chronic obstructive pulmonary disease/pulmonary hyperten-sion/interleukin-6/inflammation分类
医药卫生引用本文复制引用
侯润华,陈蕊,李德富,王思婷,章洪萍,叶园园,杨泽萍,李梦珠,郑冠钦,樊家睿,张春云..雾化吸入丹参酮ⅡA磺酸钠对小鼠慢性阻塞性肺疾病相关肺动脉高压的治疗作用[J].中国病理生理杂志,2025,41(9):1713-1720,8.基金项目
国家自然科学基金资助项目(No.81900033) （No.81900033）
广东省自然科学基金面上项目(No.2022A1515012504 （）
No.2025A1515012544) （）
广州市科技计划项目(2025A03J3227) （2025A03J3227）
