中国病理生理杂志2025，Vol.41Issue(9)：1748-1756,9.DOI:10.3969/j.issn.1000-4718.2025.09.010
基于共培养体系探讨逍遥抗癌解郁方含药血清对体外乳腺癌相关抑郁模型BV2和HT22细胞的影响
Effects of Xiaoyao-Kang'ai-Jieyu formula-containing serum on BV2-HT22 cell co-culture system from mice with breast cancer-related depression
摘要
Abstract
AIM:To investigate the effects of Xiaoyao-Kang'ai-Jieyu formula(XYKAJY)-containing serum on the co-culture of BV2 and HT22 cells from mice with breast cancer-related depression(BCRD).METHODS:The op-timal concentration of XYKAJY-containing serum was determined using mouse HT22 cells.In vitro co-culture model of mouse HT22 and BV2 cells was established through incubation with 4T1 cell supernatant and 200 µmol/L corticosterone.The cells were then divided into control group,model group,blank serum group,positive drug serum group,5%XY-KAJY-containing serum group,10%XYKAJY-containing serum group,15%XYKAJY-containing serum group and 20%XYKAJY-containing serum group.Alterations in HT22 cell viability was detected by CCK8 assay.The concentration level of interleukin-1β(IL-1β),IL-6 and IL-18 in the cell supernatant was quantified using enzyme-linked immunosorbent as-say(ELISA).Flow cytometry was conducted to assess the apoptosis rate of HT22 cells and quantify the expression levels of adhesion molecule CD11b on the surface of BV2 cells.Immunofluorescence was performed to determine the expression of neuron-specific enolase(NSE),synapsin 1(Syn1)and postsynaptic density protein 95(PSD95)in HT22 cells,and that of inducible nitric oxide synthase(iNOS)in BV2 cells.RESULTS:Treatment with XYKAJY-containing serum down-regulated CD11b expression level in BV2 cells(P<0.01),and reduced the release of IL-1β,IL-6 and IL-18(P<0.01).Moreover,it significantly improved the viability of HT22 cells(P<0.01),enhanced cell morphology integrity,and reduced the apoptosis rate(P<0.01).CONCLUSION:These findings indicated that XYKAJY-containing serum im-proved the function of mouse HT22 neurons by regulating the polarization phenotype of mouse BV2 cells and alleviating neuroinflammatory-induced toxicity.关键词
逍遥抗癌解郁方/乳腺癌相关抑郁/BV2细胞/HT22细胞/共培养/胶质细胞极化Key words
Xiaoyao-Kang'ai-Jieyu formula/breast cancer-related depression/BV2 cells/HT22 cells/co-culture/glial polarization分类
医药卫生引用本文复制引用
杨松,韩远山,邹蔓姝,余晓诗,余永红,王宇红..基于共培养体系探讨逍遥抗癌解郁方含药血清对体外乳腺癌相关抑郁模型BV2和HT22细胞的影响[J].中国病理生理杂志,2025,41(9):1748-1756,9.基金项目
国家自然科学基金青年科学基金项目(No.82104846) （No.82104846）
湖南省卫健委一般项目(No.w20243097) （No.w20243097）
中药粉体与创新药物研究省部共建国家重点实验室培育基地开放基金项目(No.24PTKF1013) （No.24PTKF1013）
