中国草地学报2025，Vol.47Issue(9)：1-15,15.DOI:10.16742/j.zgcdxb.20250062
盐胁迫下紫花苜蓿活性氧清除相关酶基因的鉴定与分析
Identification and Analysis of Genes Related to Reactive Oxygen Species Scavenging Enzymes in Alfalfa under Salt Stress
摘要
Abstract
Salt stress leads to the accumulation of reactive oxygen species(ROS)in plants,causing dam-age to plant cells.However,the enzymatic antioxidant system is responsible for scavenging ROS in plant cells and protecting cells from ROS damage.At present,there are few functional analyses of genes related to ROS-scavenging enzymes in alfalfa(Medicago sativa)in response to salt stress.Based on the transcriptome data of roots and leaves of salt-tolerant alfalfa cultivar(GIB)and salt-sensitive cultivar(LS)under salt stress,this study identified 28 differentially expressed genes related to ROS-scavenging.Twenty-eight genes are distrib-uted on 19 chromosomes,belonging to five gene families:MsGST,MsPOD,MsAOX,MsGRX and MsPrdx,among which MsGST(12)and MsPOD(11)families account for the largest proportion.The physi-cochemical properties of ROS scavenging enzyme proteins were different among the members of each gene fam-ily.There were highly similar conserved motifs in the members of the gene family.Phylogenetic analysis showed that MsGSTUs and MsPODs were located in the branches defined by Tau and AtPERs in Arabidopsis thaliana,respectively,suggesting that they had similar evolutionary processes and similar functions.Real-time fluorescence quantitative PCR analysis showed that the expression levels of MsGSTU26,MsPOD2 and MsPOD4 genes in GIB leaves under salt stress were significantly higher than those in LS,and the promoter regions of the three genes had elements(G-box and CGTCA-motif)related to adverse stress.It indicated that the MsGSTU26,MsPOD2 and MsPOD4 genes may be involved in the regulation of the adaptability of GIB and LS to salt stress.关键词
紫花苜蓿/盐胁迫/活性氧清除酶/生物信息学分析/实时荧光定量PCRKey words
Alfalfa/Salt stress/ROS-scavenging enzyme/Bioinformatics analysis/Real-time quanti-tative PCR分类
农业科技引用本文复制引用
余如刚,杨改梅,韦英铭,杜雪玲,王国良,陈鑫,杨琳..盐胁迫下紫花苜蓿活性氧清除相关酶基因的鉴定与分析[J].中国草地学报,2025,47(9):1-15,15.基金项目
安徽省高校自然科学研究重点项目(2022AH050385) （2022AH050385）
财政部和农业农村部:国家现代农业产业技术体系(CARS-34) （CARS-34）
山东省牛产业技术体系(SDAIT-9-10) （SDAIT-9-10）
校级科学研究项目(2024ZK38) （2024ZK38）
国家级大学生创新创业训练计划项目(202310373031) （202310373031）
