中国农业科技导报2025，Vol.27Issue(10)：51-62,12.DOI:10.13304/j.nykjdb.2024.0301
亚适低温对生殖期草莓叶片结构和光合荧光特性的影响
Effects of Suboptimal Low Temperatures on Leaf Structure and Photosynthetic Fluorescence Characteristics of Strawberry During Reproductive Period
摘要
Abstract
In order to explore the adaptation mechanism of strawberries under suboptimal low temperature,2 strawberry varieties of Ningyu and Jingzangxiang were taken as materials.The phenotype traits,photosynthetic parameters,rapid chlorophyll fluorescence parameters and leaf ultrastructure of strawberry leaves under different treatments during the reproductive period were determined.The results showed that,under suboptimal low temperature,there was no significant change on plant-type of Ningyu,while the plant-type of Jingzangxiang gradually opened from the intermediate type and the leaf color gradually changed to yellow green.With the prolongation of suboptimal low temperature,the leaf area,leaf thickness and leaf volume of Ningyu significantly decreased,the leaf tissue density significantly increased,the specific leaf area first increased and then decreased,and the leaf dry matter content firstly decreased and then increased.With the prolongation of suboptimal low temperature,the leaf area and volume of Jingzangxiang increased,while the leaf thickness decreased,the leaf tissue density and leaf dry matter content first decreased and then increased,the specific leaf area and density showed a trend of first increasing and then decreasing.With the prolongation of suboptimal low temperature,there was no significant change in chlorophyll content in leaves of Ningyu,however,the chlorophyll a content of Jingzangxiang increased significantly.The net photosynthetic rate,stomatal conductance and intercellular CO2 concentration of Ningyu leaves all significantly increased after 7 d of stress,and decreased after 14 d of stress.Suboptimal low temperature led to a decrease in water use efficiency,although it increased after 14 d of stress,it was still lower than the control at the same period.For Jingzangxiang,the net photosynthetic rate of leaves significantly decreased after 14 d of stress,while the intercellular CO2 concentration and stomatal conductance significantly increased after 7 d of stress and decreased after 14 d of stress,the transpiration rate under suboptimal low temperature treatment decreased after 14 d of stress,and the overall trend of water use efficiency was similar to that of Ningyu.Energy transfer of Ningyu leaves was hindered after 14 d of suboptimal low temperature treatment(ΔWOK>0),however,the Jingzangxiang had already been hindered after 7 d of stress.The suboptimal low temperature had a significant impact on the oxygen release complexes of Ningyu and Jingzangxiang,and both oxygen release complexes were inactivated(ΔWOJ>0).The leaf mesophyll cells of Ningyu and Jingzangxiang under control treatment were intact,and the chloroplast and other structures were intact and clear.Under suboptimal low temperature treatment,the mitochondria in the mesophyll cells of Ningyu were slightly swollen,and a few rough endoplasmic reticulum were slightly dilated.After 14 d of stress,the lamellae and stacking structure of chloroplasts became blurred.After 7 d of stress,Jingzangxiang showed slight cytoplasmic wall separation,chloroplast structure dissolution,unclear outer membrane boundary,and disordered arrangement of lamellar structure.After 14 d of stress,chloroplasts dissolved and denatured,and lamellar structure became blurred.In summary,the leaves of strawberry should adjust under suboptimal low temperature treatment,but long-term suboptimal low temperature could reduce the photosynthetic performance of the leaves and damage the leaf ultrastructure.The damage caused by suboptimal low temperature to the photosystemⅡ reaction center and chloroplasts of Jingzangxiang was greater than that of Ningyu.The plant status,photosynthetic performance and other indicators of Ningyu were relatively stable,and its ability to low temperature was stronger than Jingzangxiang.关键词
草莓/叶结构型性状/光合/亚适低温/叶超微结构Key words
strawberry/leaf structural traits/photosynthesis/suboptimal low temperature/leaf ultrastructure分类
农业科技引用本文复制引用
杨馥霞,贺欢,汤玲,李宽莹,刘芬,陈建军..亚适低温对生殖期草莓叶片结构和光合荧光特性的影响[J].中国农业科技导报,2025,27(10):51-62,12.基金项目
甘肃省农业科学院重点研发计划项目(2025GAAS33) （2025GAAS33）
甘肃省科技厅民生科技专项(21CX6NA120) （21CX6NA120）
甘肃省农业科学院科研结余资金项目(2024LGS08) （2024LGS08）
甘肃省农业科学院重点研发计划项目(2022GAAS42) （2022GAAS42）
甘肃省青年科技基金计划项目(20JR10RA461). （20JR10RA461）
