中国农业科技导报2025，Vol.27Issue(10)：63-71,9.DOI:10.13304/j.nykjdb.2024.0215
低温胁迫对'WL'系列苜蓿品种碳氮物质含量和相对电导率的影响
Effects of Low Temperature Stress on Non-structural Carbon and Nitrogen Content and Relative Conductivity of'WL'Alfalfa Varieties
摘要
Abstract
In order to explore the differences in cold resistance among different alfalfa varieties under low temperature stress and the changes in the contents of non-structural carbon and nitrogen substances in the roots,the overwintering organs(alfalfa roots)of 12 different fall dormancy alfalfa varieties of the'WL'series planted in Horqin sandy land were dug before overwintering,and the programmable constant temperature and humidity test box was used to simulate the low temperature refrigeration(-20℃)treatment,and the low temperature cold storage(4℃)treatment was used as the control.The relative conductivity and non-structural carbon and nitrogen substance content of alfalfa root neck were measured,and correlation,clustering,principal component and membership function analyses were conducted.The results showed that the relative conductivity,soluble sugar,soluble protein and free amino acid contents under low temperature refrigeration increased compared to the control,while the starch content decreased.Correlation analysis showed that under low-temperature refrigeration,the relative conductivity of alfalfa root neck was significantly negatively correlated with starch content and significantly positively correlated with soluble protein content(P<0.05);under low temperature refrigeration,the relative conductivity of alfalfa root neck was significantly negatively correlated with starch and soluble protein contents(P<0.05).Cluster analysis divided 12 different fall dormancy levels of'WL'series into 2 categories.The first category consisted of 3 autumn dormant alfalfa varieties WL168HQ,WL298HQ and WL319HQ.The second category consisted of 1 autumn dormant alfalfa varieties WL329HQ and 8 semi autumn dormant alfalfa varieties.The comprehensive evaluation of the affiliation function showed that the order of the strength of cold resistance of different fall dormancy alfalfa was as follows:WL319HQ>WL298HQ>WL168HQ>WL349HQ>WL329HQ>WL354HQ>WL343HQ>WL358HQ>WL366HQ>WL377HQ>WL363HQ>WL440HQ.In summary,it was suggested to plant low autumn dormant alfalfa varieties with strong cold resistance such as WL168HQ,WL319HQ,WL298HQ in Horqin sandy land.关键词
低温胁迫/苜蓿/相对电导率/抗寒性Key words
low temperature stress/alfalfa/relative conductivity/cold resistance分类
农业科技引用本文复制引用
隋可,刘泽宇,张玉霞,刘庭玉,单新河,张智勇,王显国..低温胁迫对'WL'系列苜蓿品种碳氮物质含量和相对电导率的影响[J].中国农业科技导报,2025,27(10):63-71,9.基金项目
国家牧草产业技术体系建设项目(CARS-34) （CARS-34）
内蒙古自治区直属高校专项(GXKY22018) （GXKY22018）
内蒙古自治区生态农业国家民委重点实验室开放基金项目(MDK2023095) （MDK2023095）
中央支持地方高校改革发展-作物学(草学)提质学科建设项目(240101). （草学）
