中国农业科技导报2025，Vol.27Issue(10)：72-83,12.DOI:10.13304/j.nykjdb.2024.0297
蛇足石杉YUCCA基因家族的生物信息学及其组织特异表达分析
Bioinformatics and Tissue-specific Expression Analysis of YUCCA Gene Family in Huperzia serrata
摘要
Abstract
The YUCCA gene encodes flavin monooxygenase,which plays an important regulatory role in plant growth and development.In order to explore the effect of YUCCA gene on the growth and development of Huperzia serrata,bioinformatics and tissue-specific expression analysis of YUCCA gene family of H.serrata were carried out.The results showed that 18 members of the HsYUCCA gene family were identified in the full-length transcriptome of H.serrata.The amino acid length was 150～544 aa,the relative molecular weight of the protein was 16 105.53～59 058.24 Da,and the isoelectric point was 4.36～9.63.Among them,there were 9 basic proteins,4 stable proteins,15 hydrophilic proteins,and 7 proteins with transmembrane structure.The subcellular localization of HsYUCCA were in the nucleus,cell membrane,cytoplasm,mitochondria,chloroplast and peroxisome.The expression of HsYUCCA4 was the highest in spores,stems and spores,and the expression of HsYUCCA6 was the highest in leaves.HsYUCCA had 35 cis-acting elements such as hormone response,light response and stress response,and had 15 conserved motifs.The physical and chemical properties,subcellular localization,secondary structure,tertiary structure and conserved motifs of HsYUCCA1,HsYUCCA2 and HsYUCCA4,HsYUCCA5 and HsYUCCA6,HsYUCCA7 and HsYUCCA13 were similar,indicating that their biological functions were similar,and the phylogenetic development showed certain homology,and the evolutionary process tended to be consistent.Above results provided a theoretical basis for the future analysis of the possible mechanism of YUCCA gene in the growth,development and reproduction of H.serrata.关键词
蛇足石杉/YUCCA基因家族/生物信息学/表达分析Key words
Huperzia serrata/YUCCA gene family/bioinformatics/expression analysis分类
农业科技引用本文复制引用
陈秋洁,黄玉妹,冼金燕,李凯童,黄杰兰,石小秀,王柳萍,涂冬萍..蛇足石杉YUCCA基因家族的生物信息学及其组织特异表达分析[J].中国农业科技导报,2025,27(10):72-83,12.基金项目
广西壮族自治区自然科学基金项目(2017GXNSFBA198094) （2017GXNSFBA198094）
广西壮族自治区科技基地和人才专项(2021AC19431) （2021AC19431）
广西中医药大学桂派中医药传承创新团队项目(2022B005) （2022B005）
广西中医药大学桂派杏林青年英才项目(2022C033). （2022C033）
