中国农业科技导报2025，Vol.27Issue(10)：84-94,11.DOI:10.13304/j.nykjdb.2024.0747
SNF1与MetK1共表达促进酵母S-腺苷-L-甲硫氨酸合成
Co-expression of SNF1 and MetK1 Promoted S-adenosine-L-methionine Synthesis in Saccharomyces cerevisiae
摘要
Abstract
S-adenosyl-L-methionine(SAM)is an important bioactive molecule in all living organisms.Regulating the glucose effect of Saccharomyces cerevisiae and directing the carbon flux to SAM synthesis is a challenge for cell factory.The regulating glucose effect by overexpressing SNF1 and introducing SAM synthase gene MetK1 of Leishmania infantum were applied to improve SAM production in S.cerevisiae CGMCC 2842(2842).The results showed that the overexpression of SNF1 enhanced the expression levels of genes involved in glucose transport and glycolysis,which improved the glucose utilization and then elevated the levels of glycolytic intermediates.The expression levels of ACS1 and ALD6 and the activity of alcohol dehydrogenase 2(ADH2)were enhanced,which probably promoted the conversion of ethanol in fermentation broth into acetyl-CoA.The gene expressions involved in sulfur-containing amino acids were also enhanced for the precursor amino acid biosynthesis.Finally,the introduction of MetK1 of L.infantum effectively directed the carbon metabolism flux to SAM synthesis.The SAM production of the stain YPSNF1-MetK1 reached 1.90 g·L-1,increased by 251.85%compared to that of 2842.Above results laid theoretical foundation for the regulation and application of glucose effect in yeast.关键词
酿酒酵母/葡萄糖效应/S-腺苷甲硫氨酸/SNF1/MetK1Key words
Saccharomyces cerevisiae/glucose effect/S-adenosyl-L-methionine/SNF1/MetK1分类
生物科学引用本文复制引用
陈海龙,戴佳良,邓晶,高新星,朱官兴,何清明,朱年青..SNF1与MetK1共表达促进酵母S-腺苷-L-甲硫氨酸合成[J].中国农业科技导报,2025,27(10):84-94,11.基金项目
江苏高校"青蓝工程"资助项目 （）
江苏省高校重点实验室开放课题项目(SX1702) （SX1702）
泰州市科技支撑计划农业发展项目(TN202415) （TN202415）
泰州学院2023年度教育教学改革研究项目(2023JGB11). （2023JGB11）
