中国农业科学2025，Vol.58Issue(17)：3434-3450,17.DOI:10.3864/j.issn.0578-1752.2025.17.006
水稻细菌性条斑病菌拮抗放线菌的鉴定及其防病和促生功能
Identification and Evaluation of Biocontrol Actinomycetes Against Xanthomonas oryzae pv.oryzicola for Disease Suppression and Growth Promotion in Rice
摘要
Abstract
[Objective]Rice bacterial leaf streak(BLS)is a quarantine-regulated bacterial disease in China caused by Xanthomonas oryzae pv.oryzicola(Xoc),which has severely threatened rice yield and grain quality,becoming one of the main diseases in rice production areas.This study aims to utilize high-quality actinomycete strains to lay a theoretical foundation for developing the products of microbial origin to mitigate crop diseases.[Method]Actinomycete strains were isolated and purified from rhizosphere soil samples of various plants using the serial dilution plating technique.The inhibition ability of different strains against Xoc was compared by measuring the diameter of the inhibition zone.The target strain with the best inhibition effect was selected for preservation and identified through polyphasic characterization integrating morphological traits,physiological-biochemical experiments,and multi-gene alignment analysis.The effects of antagonistic actinomycete on the physiological characteristics of Xoc were investigated by scanning electron microscopy,Fourier-transform infrared spectroscopy(FTIR),intracellular β-galactosidase leakage determination and SDS-PAGE gel electrophoresis.The greenhouse pot control effect test was carried out to study the actual benefit of controlling BLS.The growth-promoting characteristics of antagonistic actinomycete were analyzed by designated medium,and its effects on the growth and development of rice seedlings were investigated by watering growth-promoting experiments.[Result]A total of 80 actinomycete strains were isolated.Among them,strain Sv-6 exhibited the most potent antagonism against Xoc,with inhibition zone diameter of(44.87±0.26)mm.Based on the morphological characteristics and phylogenetic analysis,strain Sv-6 was identified as Streptomyces virginiae.After treatment with the culture filtrate of Sv-6 strain,Xoc cells swelled,shrunk and aggregated.At the same time,the material composition of the membrane surface changed,the permeability increased,and the protein expression decreased.The results of greenhouse pot experiments showed that the lesion inhibition rates of susceptible rice varieties Yongyou 15 and Xiangliangyou 900 were 57.98％-88.25％after being treated with strain Sv-6 culture fluid,which exhibited a good preventive effect on rice infected with Xoc.The growth-promoting characterization confirmed that strain Sv-6 exhibited siderophore production,inorganic phosphate solubilization,and IAA production.The growth-promoting irrigation experiments confirmed that strain Sv-6 enhanced the growth of rice seedlings,and the root length increased by 48.50％after treatment.[Conclusion]S.virginiae Sv-6 exhibits a good control effect on BLS,and has the potential to be developed into green biocontrol agent and microbial fertilizer.关键词
水稻细菌性条斑病/稻黄单胞杆菌稻生致病变种/弗吉尼亚链霉菌/生物防治/促生评价Key words
rice bacterial leaf streak(BLS)/Xanthomonas oryzae pv.oryzicola(Xoc)/Streptomyces virginiae/biological control/growth-promoting evaluation引用本文复制引用
胡佳燕,沈之涵,温丽慧,郁嘉豪,章雨君,蒋冬花..水稻细菌性条斑病菌拮抗放线菌的鉴定及其防病和促生功能[J].中国农业科学,2025,58(17):3434-3450,17.基金项目
浙江省基础公益研究计划(LGN22C140004)、金华市科技计划(2023-4-020)、2025年浙江省大学生科技创新活动计划(新苗人才计划)(2025R404A035) （LGN22C140004）
