中国农业科学2025，Vol.58Issue(17)：3488-3502,15.DOI:10.3864/j.issn.0578-1752.2025.17.010
草莓连作对土壤微生物及碳氮磷功能基因丰度的影响
Effects of Strawberry Continuous Cropping on Soil Microbial Community Composition and Carbon,Nitrogen and Phosphorus Metabolism Gene Abundance
摘要
Abstract
[Objective]This paper aimed to investigate the characteristic of soil microorganisms and soil function under long-term strawberry continuous cropping and to clarify the effects of long-term strawberry continuous cropping on soil bacterial and fungal community structure and carbon,nitrogen and phosphorus metabolism gene abundance,so as to provide the scientific basis for improving the soil microecological balance and soil function of continuous cropping in the future.[Method]The real-time PCR,Miseq sequencing and high-throughput chip technologies were applied to determine soil bacteria,fungi and function under strawberry cultivated for 1,3 and 10 year.[Result]The strawberry continuous cropping reduced the soil pH,but increased the soil nutrient content,in which the soil organic matter content increased from 21.2 g·kg-1 to 32.4 g·kg-1.The bacterial abundance in rhizosphere and bulk soil was increased and then decreased as the years of cultivation.The abundance of bulk soil fungi was similar to the trend of bacteria,but its abundance was significantly reduced in the rhizosphere,indicating that bacteria and fungi response differently to continuous cropping.Continuous cropping had no significant effect on bacterial diversity,but significantly reduced fungal diversity and significantly changed soil microbial composition.Based on UniFrac distance,it was found that the fungal community UniFrac distance(0.64-1.36)was much higher than the bacteria(0.028-0.111),indicating that the influence of continuous cropping on fungal community structure was higher than that of bacteria.Correlation analysis showed that bacterial community structure was significantly correlated with soil pH,while fungal community structure was significantly correlated with soil nutrient status(such as soil available P,alkali-hydrolysable,and soil organic matter).Long-term continuous cropping of strawberry changed the metabolic function gene abundance of soil carbon,nitrogen and phosphorus,which significantly reduced the soil carbon fixation gene accA,while the nitrogen fixation gene nifH and phosphorus metabolism related functions(phoD,phoX and pqqC genes)first increased and then decreased.The partial least squares path model(PLS-PM)analysis showed that the fungal community structure(abundance,diversity and composition)caused by long-term strawberry continuous cropping had a higher impact on soil carbon,nitrogen and phosphorus metabolism gene abundance than the bacterial community structure.[Conclusion]This study showed that soil function gene abundance changed caused by long-term strawberry continuous cropping was mainly caused by the changes of fungal community structure.Thus,the soil fungal community structure should be regulated to improve the health status of long-term continuous cropping soil.关键词
草莓/连作/群落组成/细菌和真菌/土壤功能基因Key words
strawberry/continuous cropping/community composition/bacteria and fungi/soil microbial functional genes引用本文复制引用
王庆峰,褚长彬,赵峥,吴淑杭,周德平..草莓连作对土壤微生物及碳氮磷功能基因丰度的影响[J].中国农业科学,2025,58(17):3488-3502,15.基金项目
上海市科委农业领域项目(23N61900200)、上海市青年科技英才扬帆计划(20YF1442600)、上海市农业科学院卓越团队建设计划[沪农科卓[2022]008]、上海市科委农业领域重点攻关项目(18391902600) （23N61900200）
