中国蔬菜Issue(9)：74-81,8.DOI:10.19928/j.cnki.1000-6346.2025.3024
大蒜As6G-FFT基因结构与表达特性分析
Analysis of the Gene Structure and Expression Characteristics of As6G-FFT in Garlic
摘要
Abstract
Fructan:fructan 6G-fructosyltransferase(6G-FFT)is an important gene in garlic fructan metabolism,which was involved in stress response process of plants.In order to understand the gene structure and expression characteristics of As6G-FFTin depth,in this study,the genomic DNA(gDNA)and promoter sequences of As6G-FFT were selected through garlic genome.The sequences characteristics were analyzed,and regulation role of exogenous hormones to As6G-FFT was verified.The results showed that As6G-FFT was 7 943 bp in length,containing 9 exons and 8 introns.The promoter sequence contained various stress response elements and hormone response elements,such as,Salicylic acid(SA)and Abscisic acid(ABA).The qRT-PCR results confirmed that the expression of As6G-FFT was induced by SA and the induction was tissue-specific.Under normal growth conditions with exogenous salicylic acid(SA)treatment,As6G-FFTin leaves responded rapidly in the earlier stage(2 h)of the SA treatment,while the expression of As 6G-FFT in bulbs peaked in the late stage(9 d)of the SA treatment.Under drought stress,exogenous SA significantly induced the expression of As6G-FFTin both roots and bulbs,and the inducing effect was more pronounced than that of bulbs.Under low-temperature stress,exogenous SA only had an inducing effect on the expression of As6G-FFT in bulbs.Therefore,it is hypothesized that garlic bulbs would be an important tissue for As6G-FFT to mediate SA regulation in response to drought and low temperature stress.关键词
As6G-FFT/启动子/顺式作用元件/基因表达Key words
As6G-FFT/promoter/cis acting element/gene expression引用本文复制引用
卫晨龙,周倩怡,余小奎,闫艺薇,赵佳璐,田洁..大蒜As6G-FFT基因结构与表达特性分析[J].中国蔬菜,2025,(9):74-81,8.基金项目
青海省中央引导地方科技发展资金项目(2024ZY044) （2024ZY044）
