中国兽医科学2025，Vol.55Issue(9)：1137-1143,7.DOI:10.16656/j.issn.1673-4696.2025.0175
细胞核糖体蛋白S5对猪流行性腹泻病毒复制的抑制作用
Inhibition of porcine epidemic diarrhea virus replication by ribosomal protein S5 of cell
摘要
Abstract
This study was aimed to explore the effect of ribosomal protein S5(RPS5)of cell on porcine epidemic diarrhea virus(PEDV)replication.The fluorescence quantitative PCR,Western-blot,and other methods were performed to determine the dynamic changes of RPS5 transcription and translation levels and the effects on PEDV replication at different times after PEDV infection.The results showed that the transcription and RPS5 protein expression levels in Marc-145 and Vero cells decreased after PEDV in-fection.Compared with the control group,overexpression of RPS5 significantly inhibited PEDV replica-tion,but enhanced PEDV replication significantly after the transfection with siRPS5.RPS5 did not af-fect the PEDV attachment and entry,but mainly inhibited PEDV replication and transcription,indicated the antiviral effect of RPS5,which provided theoretical basis for the prevention and control of piglet diarrhea.关键词
RPS5/PEDV/病毒复制/抗病毒作用Key words
RPS5/PEDV/viral replication/antiviral effect分类
农业科技引用本文复制引用
田朔,王祎,沈瑶歆,李宇鹏,范宝超,郭容利,商红旗,李彬..细胞核糖体蛋白S5对猪流行性腹泻病毒复制的抑制作用[J].中国兽医科学,2025,55(9):1137-1143,7.基金项目
国家自然科学基金项目(32373030,32272996) （32373030,32272996）
中国博士后基金项目(2024M751210) （2024M751210）
江苏省大型科学仪器开放共享自主研究课题项目[GX(23)2001] （23）
