中国兽医科学2025，Vol.55Issue(9)：1144-1151,8.DOI:10.16656/j.issn.1673-4696.2025.0160
口蹄疫矿化病毒样颗粒疫苗对猪免疫持续期的评价
Evaluation of immune duration of a biomineralized foot-and-mouth disease virus-like particle vaccine in swine
摘要
Abstract
This study aimed to evaluate the immune duration of a biomineralized foot-and-mouth disease virus-like particle(FMDV VLPs)vaccine.Using purified O-type FMDV VLPs antigen as the founda-tion,a calcium phosphate biomineralized protective layer was constructed on the particle surface through biomineralization.Vaccines were formulated by combining the biomineralized VLPs with ISA 201 adjuvant or water-soluble adjuvant,followed by immunization of swine.Lymphocyte proliferation,Th1/Th2 immune responses,specific antibody titers,and protection efficacy post-challenge were as-sessed to evaluate the vaccine's immune duration.Results showed that at 28 days post-immunization,both biomineralized FMDV VLPs formulated with ISA 201 adjuvant or water-soluble adjuvant induced Th1/Th2 immune responses,lymphocyte proliferation,and high-titer specific antibody production.By 210 days post-immunization,the ISA 201 adjuvant group maintained specific antibody titers above 1∶64,achieving an 80％protection rate post-challenge.This study demonstrates that biomineralized FMDV VLPs combined with ISA 201 adjuvant can provide long-lasting immune protection for at least 210 days,offering a novel strategy for developing safe and stable FMDV VLPs vaccines.关键词
口蹄疫病毒/病毒样颗粒/生物矿化/免疫持续期Key words
foot-and-mouth disease virus/virus-like particles/biomineralization/immunization duration分类
农业科技引用本文复制引用
段梦迪,敬晓棋,白满元,刘蕾,李毅,穆素雨,滕志东,张韵,丁耀忠,孙世琪,郭慧琛..口蹄疫矿化病毒样颗粒疫苗对猪免疫持续期的评价[J].中国兽医科学,2025,55(9):1144-1151,8.基金项目
国家自然科学基金项目(32473012,32301127) （32473012,32301127）
国家成都农业科技中心地方财政专项(NASC2024KR06) （NASC2024KR06）
甘肃省科技重大专项(24ZDWA004) （24ZDWA004）
甘肃省技术创新引导计划-东西部科技协作专项(24CXNA030) （24CXNA030）
宁夏重点研发计划项目(2024BBF02017) （2024BBF02017）
兰州市人才创业创新项目(2023-RC-3) （2023-RC-3）
兰州市青年科技人才创新项目(2024-QN-3) （2024-QN-3）
