中国兽医科学2025，Vol.55Issue(9)：1152-1159,8.DOI:10.16656/j.issn.1673-4696.2025.0154
沙门菌蛋白PgtE的免疫原性研究
Study on the immunogenicity of PgtE protein from Salmonella
摘要
Abstract
This study investigated the immunogenicity of Salmonella outer membrane protein PgtE.The pgte gene was cloned into pET28a,then PgtE proteins were expressed in Escherichia coli BL21,and were purified via His-tag affinity chromatography.PgtE proteins were mixed with manganese gluconate(Mn2+)and aluminum hydroxide(AH)adjuvant to immunize mice at the dosage of 50 μg per mouse.Cell proli-feration assays,enzyme-linked immunosorbent assay(ELISA),and bacterial load tests were used to evaluate the immunological effects.Compared with groups,the data showed that PgtE+Mn2++AH group signifi-cantly enhanced splenic lymphocyte proliferation(SI),elevated levels of IL-2,IL-4,IL-10,IFN-γ and IgG(P＜0.05)in serum,and reduced Salmonella loads in the spleen,liver,and kidney(P＜0.05).PgtE+Mn2++AH group and PgtE group exhibited the immunoprotection rate of 100％and 77％,respectively,which was significantly higher than PBS group.These results indicate that PgtE exhibits strong immunogenicity,Mn2+and AH adjuvant synergistically enhances its immune efficacy,providing a foundation for Salmonella subunit vaccine development.关键词
沙门菌/PgtE/原核表达/免疫原性Key words
Salmonella/PgtE/prokaryotic expression/immunogenicity分类
农业科技引用本文复制引用
岳佳颖,魏书宇,陶明阳,刘渴欣,王北艳,于立权,马金柱,宋佰芬..沙门菌蛋白PgtE的免疫原性研究[J].中国兽医科学,2025,55(9):1152-1159,8.基金项目
国家重点研发计划项目(2023YFD1802504) （2023YFD1802504）
