中国现代医学杂志2025，Vol.35Issue(18)：32-41,10.DOI:10.3969/j.issn.1005-8982.2025.18.006
葛根素联合Ag-HA涂层植入对种植体周围炎大鼠炎症水平和骨矿物质密度的影响
Effects of puerarin combined with Ag-HA coating implantation on inflammation and bone mineral density in rats with peri-implantitis
摘要
Abstract
Objective To investigate the effects of puerarin combined with silver-hydroxyapatite(Ag-HA)coating implantation on inflammations,bone mineral density(BMD),and bone biological parameters in rats with peri-implantitis,and to provide new insights and experimental evidence for the treatment of peri-implantitis by comparing the clinical manifestations,gene and protein expression of inflammatory cytokines,and BMD across different treatment groups.Methods Fifty male rats were selected for this study and induced with peri-implantitis.After successful modeling,the rats were randomly divided into three groups:the peri-implantitis group(10 rats),the Ag-HA group(20 rats),and the combination group(20 rats).The peri-implantitis group received no intervention,the Ag-HA group was treated with Ag-HA coating implantation,and the combination group received both puerarin and Ag-HA coating implantation.The rats were evaluated preoperatively and at 1,2,4,and 8 weeks postoperatively for sulcus bleeding index(SBI),gingival index(GI),probing depth(PD),serum levels of inflammatory factors,and osseointegration capacity.Periodontal tissues were collected from all three groups of rats at 8 weeks postoperatively,and mRNA levels of relevant genes were detected using real-time quantitative PCR.Western blotting was performed to assess the expression of associated proteins.Micro-CT 3D reconstruction was carried out on the rat models,and bone biological parameters were statistically analyzed and compared.Results Comparisons were made on the SBI,GI,PD,levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6,IL-1β,and alkaline phosphatase(ALP),and the bone-implant contact rate among the three groups preoperatively and at 1,2,4,and 8 weeks postoperatively.The results exhibited that these indicators were statistically significant different across the time points(P＜0.05)and among the groups(P＜0.05),where the SBI,GI,PD,and levels of TNF-α,IL-6,and IL-1β were lower and the ALP level and the bone-implant contact rate were higher in the combination group,indicating better therapeutic outcomes.The change trends of all these indicators(P＜0.05)but ALP were significantly different among the groups(P＞0.05).The relative mRNA and protein expression levels of IL-1β,CXCL12,and CXCR4 in the periodontal tissues of rats in the combination group were lower than those in the Ag-HA group(P＜0.05),while the relative mRNA and protein expression levels of osteoprotegerin(OPG)in the combination group were higher than those in the Ag-HA group(P＜0.05).The tissue mineral density,bone volume-to-total volume ratio(BV/VT),and trabecular thickness(Tb.Th)of rats in the combination group were higher than those in the Ag-HA group(P＜0.05),whereas the BMD and distance from cementoenamel junction to alveolar bone crest(CEJ-ABC)were lower in the combination group than those in the Ag-HA group(P＜0.05).Conclusion Puerarin combined with Ag-HA coating implantation significantly improves inflammation levels,GI,PD,and osseointegration capacity in peri-implantitis rats.The combination treatment demonstrates more pronounced effects in suppressing inflammation,enhancing bone density,and promoting periodontal tissue repair compared to Ag-HA coating implantation alone.Notably,the combination therapy exhibits superior effects on OPG protein expression and bone biological parameters,suggesting their potential applicability in the treatment of peri-implantitis.关键词
种植体周围炎/牙周炎/葛根素/银-羟基磷灰石/大鼠/炎症因子/骨生物学参数Key words
peri-implantitis/periodontitis/puerarin/silver-hydroxyapatite/rat/inflammatory cytokine/bone biological parameters分类
医药卫生引用本文复制引用
王磊,袁青,王倩,云璐..葛根素联合Ag-HA涂层植入对种植体周围炎大鼠炎症水平和骨矿物质密度的影响[J].中国现代医学杂志,2025,35(18):32-41,10.基金项目
山东省医药卫生科技发展项目(No:202208010182) （No:202208010182）
