中国药理学通报2025，Vol.41Issue(10)：1875-1883,9.DOI:10.12360/CPB202501077
木犀草素抑制肝星状细胞自噬缓解肝纤维化的作用及机制
Luteolin alleviates liver fibrosis by inhibiting autophagy of hepatic stellate cells
摘要
Abstract
Aim To explore the mechanism of luteolin in alleviating hepatic fibrosis.Methods C57BL/6 mice were randomly divided into the control group,CCl4 group,silybin group(100 mg·kg-1)and luteo-lin group(100 mg·kg-1).After 10-week modeling and 2-week treatment,the serum levels of aminotrans-ferase and liver histopathology were examined.Hepatic fibrosis and autophagy-related gene expression were as-sessed using immunohistochemistry and immunofluores-cence.Human hepatic stellate cell line(LX2)was cultured and divided into control,TGF-β1(10 mg·L-1),TGF-β1+silybin(40 μmol·L-1),TGF-β1+luteolin(40 μmol·L-1).Fibrotic and autophagy-re-lated markers were analyzed using quantitative real-time PCR,Western blot,immunofluorescence and MDC staining.Results Compared with the CCl4 group,the treatment groups showed significantly improved liver function and reduced hepatic fibrosis,with markedly downregulated COL1A1 and α-SMA expression,and luteolin demonstrated superior efficacy.Compared with TGF-β1 group,luteolin treatment significantly de-creased mRNA levels of COL1A1,ACTA2 and MAP1LC3B,while increasing the mRNA level of SQSTM1,the protein levels of COL1A1 and α-SMA de-creased,p62 was enhanced,the LC3Ⅱ/Ⅰ ratio was downregulated,and autophagy was reduced.These effects of luteolin were reversed by autophagy inducer rapamycin.Conclusion Luteolin alleviates liver fi-brosis by decreasing the autophagy of hepatic stellate cells.关键词
木犀草素/肝纤维化/肝星状细胞/p62/LC3/雷帕霉素Key words
luteolin/liver fibrosis/hepatic stellate cells/p62/LC3/rapamycin分类
医药卫生引用本文复制引用
陈姝伶,王曦旋,李瑞祺,杨大为,曹辉,杨永峰..木犀草素抑制肝星状细胞自噬缓解肝纤维化的作用及机制[J].中国药理学通报,2025,41(10):1875-1883,9.基金项目
江苏省传染病医学创新中心资助项目(No CXZX202232) （No CXZX202232）
